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cDNA and ORF Clones: Versatile Tools for Gene Overexpression
cDNA and ORF clones are efficient and convenient resources for accessing gene content, enabling a wide range of overexpression applications in cell culture and in vitro studies. These sequences are inserted into expression vectors driven by strong promoters and delivered to target cells through plasmid transfection or lentiviral transduction.
cDNA Clones
- Preserve native regulatory elements, making them ideal for studying gene function in its natural context.
- Commonly used for overexpression studies where maintaining native regulation is essential.
ORF Clones
- Exclude 5′ and 3′ untranslated regions (UTRs), offering a direct and simplified route to protein expression.
- Provide a streamlined approach for applications requiring efficient protein production.
Applications
Both cDNA and ORF clones are widely utilized in various research areas, including:
- Gene overexpression studies.
- RNAi rescue experiments.
- Structural and functional protein analysis.
- Protein localization studies using visualization techniques.
- Biochemical purification workflows.
- Identification of protein-protein interactions through co-immunoprecipitation.
cDNA & ORF Collections
Our extensive cDNA and ORF collections are available as individual clones or in various library formats, tailored to suit diverse research needs.
Mammalian Gene Collection (MGC)
Recognized as the gold standard for full-length cDNA sequences, the Mammalian Gene Collection offers fully sequenced cDNA clones for human, mouse, rat, and bovine genes.
CCSB-Broad Lentiviral Expression Library
This library provides human ORFs in lentiviral expression vectors equipped with a C-terminal V5 tag, ideal for protein expression and interaction studies.
ORFeome Collaboration
The ORFeome Collaboration offers sequence-verified human ORFs subcloned into Gateway® recombinational entry vectors, allowing seamless integration into various experimental systems.
CCSB hORFeome Libraries
- Version 8.1: The latest release of the CCSB Human ORFeome library, developed by the Dana-Farber Cancer Institute, offering unparalleled coverage and precision.
Non-Mammalian cDNA & ORFs
Expand your research with cDNA and ORF clones for non-mammalian species, including:
- Yeast
- E. coli
- C. elegans
- D. rerio
- Xenopus