{"id":1803,"date":"2024-04-04T09:50:02","date_gmt":"2024-04-04T09:50:02","guid":{"rendered":"https:\/\/skyangbio.com\/wordpress\/?page_id=1803"},"modified":"2024-04-14T05:24:13","modified_gmt":"2024-04-14T05:24:13","slug":"orf","status":"publish","type":"page","link":"https:\/\/skyangbio.com\/wordpress\/orf\/","title":{"rendered":"ORF"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-page\" data-elementor-id=\"1803\" class=\"elementor elementor-1803\" data-elementor-post-type=\"page\">\n\t\t\t\t<div class=\"elementor-element elementor-element-877fa6b e-flex e-con-boxed e-con e-parent\" data-id=\"877fa6b\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t<div class=\"elementor-element elementor-element-47f2abc e-con-full e-flex e-con e-child\" data-id=\"47f2abc\" data-element_type=\"container\">\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-b8920ef e-con-full e-flex e-con e-child\" data-id=\"b8920ef\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-67e66c9 elementor-widget elementor-widget-heading\" data-id=\"67e66c9\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h1 class=\"elementor-heading-title elementor-size-default\">ORF<\/h1>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-b32eee5 e-flex e-con-boxed e-con e-parent\" data-id=\"b32eee5\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-1f1112e elementor-tabs-view-horizontal elementor-widget elementor-widget-tabs\" data-id=\"1f1112e\" data-element_type=\"widget\" data-widget_type=\"tabs.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-tabs\">\n\t\t\t<div class=\"elementor-tabs-wrapper\" role=\"tablist\" >\n\t\t\t\t\t\t\t\t\t<div id=\"elementor-tab-title-3251\" class=\"elementor-tab-title elementor-tab-desktop-title\" aria-selected=\"true\" data-tab=\"1\" role=\"tab\" tabindex=\"0\" aria-controls=\"elementor-tab-content-3251\" aria-expanded=\"false\">ORF clones<\/div>\n\t\t\t\t\t\t\t\t\t<div id=\"elementor-tab-title-3252\" class=\"elementor-tab-title elementor-tab-desktop-title\" aria-selected=\"false\" data-tab=\"2\" role=\"tab\" tabindex=\"-1\" aria-controls=\"elementor-tab-content-3252\" aria-expanded=\"false\">Design<\/div>\n\t\t\t\t\t\t\t\t\t<div id=\"elementor-tab-title-3253\" class=\"elementor-tab-title elementor-tab-desktop-title\" aria-selected=\"false\" data-tab=\"3\" role=\"tab\" tabindex=\"-1\" aria-controls=\"elementor-tab-content-3253\" aria-expanded=\"false\">ORF library<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t\t<div class=\"elementor-tabs-content-wrapper\" role=\"tablist\" aria-orientation=\"vertical\">\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-tab-title elementor-tab-mobile-title\" aria-selected=\"true\" data-tab=\"1\" role=\"tab\" tabindex=\"0\" aria-controls=\"elementor-tab-content-3251\" aria-expanded=\"false\">ORF clones<\/div>\n\t\t\t\t\t<div id=\"elementor-tab-content-3251\" class=\"elementor-tab-content elementor-clearfix\" data-tab=\"1\" role=\"tabpanel\" aria-labelledby=\"elementor-tab-title-3251\" tabindex=\"0\" hidden=\"false\"><h2>ORF Clones<\/h2><h5>Gateway-adapted,sequence verified<\/h5><p>MGC premier ORF Collections provide a comprehensive\u00a0resource of fully annotated, sequence-verified entry\u00a0ORFs derived from collections generated by the Dana Farber Cancer Institute-Center for Cancer Systems Biology (in collaboration with the Broad Institute) and the international ORFeome Collaboration (OC). The ORF clones are provided in Gateway adapted entry vectors for fast and convenient transfer to any compatible expression vector. Open Reading Frame (ORF) clones contain only the protein coding sequence allowing for the addition of fusion tags.<\/p><h5>Product Highlights:<\/h5><ul><li>Gateway entry vectors \u2013 easily transfer to any compatible expression vector<\/li><li>Available with and without stop codons<\/li><li>Available as individual clones, gene family and pathway libraries, whole genome libraries and custom rearrays<\/li><li>Expression-ready cloning vectors available with multiple fusion tag options to create your expression-ready tagged ORF<\/li><li>Custom cloning and lentiviral packaging services available<\/li><\/ul><h5>Lentiviral ORF Clones \u2013 Expression ready, V5 tagged<\/h5><p>MGC premier human lentiviral ORF collection was developed through the collaborative efforts of Dana-Farber Cancer Institute, The Broad Institute and the Center for Cancer Systems Biology (CCSB). For added convenience the lentiviral ORF expression vector was created to enable expression of a protein of interest with a V5 fusion tag for western blot detection, puri\u00adfication, co-immunoprecipitation, protein localization and FACS analysis.<\/p><ul><li>Ready-to-express with a V5 tag<\/li><li>Fully sequenced, with a perfect match to its parent accession.<\/li><li>Lentiviral particle formats available<\/li><li>Human genome<\/li><li>Blasticidin resistance marker may be used in combination with vectors using the more common puromycin marker<\/li><li>Custom cloning service available for mouse and rat species<\/li><\/ul><div><h2>Documents<\/h2><h5>Technical Manual<\/h5><p style=\"border: 0px; padding: 0px; color: #444444; font-family: Roboto;\"><a style=\"border: 0px; margin: 0px; padding: 0px; transition: all 0.2s ease-in 0s; color: #222222;\" href=\"http:\/\/www.moldiag.in\/wp-content\/uploads\/2016\/06\/MGC-premier-cDNA-and-ORF-Technical-manual.pdf\" target=\"_blank\" rel=\"noopener\"><span style=\"border: 0px; margin: 0px; padding: 0px; color: #1563a1;\"><strong style=\"border: 0px; margin: 0px; padding: 0px;\">MGC\u00a0premier\u00a0Tagged ORFs Clones \u2013 Technical Manual<\/strong><\/span><\/a><\/p><p style=\"border: 0px; padding: 0px; color: #444444; font-family: Roboto;\"><a style=\"border: 0px; margin: 0px; padding: 0px; transition: all 0.2s ease-in 0s; color: #222222;\" href=\"http:\/\/www.moldiag.in\/wp-content\/uploads\/2016\/06\/MGC-premier-Lentiviral-V5-Tagged-ORFs-Glycerol-stock-Technical-Manual-v02.pdf\" target=\"_blank\" rel=\"noopener\"><span style=\"border: 0px; margin: 0px; padding: 0px; color: #1563a1;\"><strong style=\"border: 0px; margin: 0px; padding: 0px;\">MGC\u00a0premier\u00a0Lentiviral V5-Tagged ORFs (Glycerol Stock) \u2013 Technical Manual<\/strong><\/span><\/a><\/p><p style=\"border: 0px; padding: 0px; color: #444444; font-family: Roboto;\"><a style=\"border: 0px; margin: 0px; padding: 0px; transition: all 0.2s ease-in 0s; color: #222222;\" href=\"http:\/\/www.moldiag.in\/wp-content\/uploads\/2016\/06\/MGC-premier-Lentiviral-V5-Tagged-ORFs-Lentiviral-particles-Technical-Manual.pdf\" target=\"_blank\" rel=\"noopener\"><span style=\"border: 0px; margin: 0px; padding: 0px; color: #1563a1;\"><strong style=\"border: 0px; margin: 0px; padding: 0px;\">MGC premier Lentiviral V5-Tagged ORFs (Viral Particles) \u2013 Technical Manual<\/strong><\/span><\/a><\/p><p style=\"border: 0px; padding: 0px; color: #444444; font-family: Roboto;\"><a style=\"border: 0px; margin: 0px; padding: 0px; transition: all 0.2s ease-in 0s; color: #222222;\" href=\"http:\/\/www.moldiag.in\/wp-content\/uploads\/2016\/06\/TOH3507-Human-ORFeome-Collaboration-Library-Technical-Manual.pdf\" target=\"_blank\" rel=\"noopener\"><span style=\"border: 0px; margin: 0px; padding: 0px; color: #1563a1;\"><strong style=\"border: 0px; margin: 0px; padding: 0px;\">MGC\u00a0premier\u00a0Human ORFeome Collaboration Library \u2013 Technical Manual<\/strong><\/span><\/a><\/p><p style=\"border: 0px; padding: 0px; color: #444444; font-family: Roboto;\"><a style=\"border: 0px; margin: 0px; padding: 0px; transition: all 0.2s ease-in 0s; color: #222222;\" href=\"http:\/\/www.moldiag.in\/wp-content\/uploads\/2016\/06\/TOH3506-Human-ORFeome-V8-1-Technical-Manual.pdf\" target=\"_blank\" rel=\"noopener\"><span style=\"border: 0px; margin: 0px; padding: 0px; color: #1563a1;\"><strong style=\"border: 0px; margin: 0px; padding: 0px;\">MGC\u00a0premier\u00a0Human ORFeome V8.1 Library \u2013 Technical Manual<\/strong><\/span><\/a><\/p><p style=\"border: 0px; padding: 0px; color: #444444; font-family: Roboto;\"><a style=\"border: 0px; margin: 0px; padding: 0px; transition: all 0.2s ease-in 0s; color: #222222;\" href=\"http:\/\/www.moldiag.in\/wp-content\/uploads\/2016\/06\/TOH7500-MGC-premier-Lentiviral-ORF-Expression-Library-Technical-Manual.pdf\" target=\"_blank\" rel=\"noopener\"><span style=\"border: 0px; margin: 0px; padding: 0px; color: #1563a1;\"><strong style=\"border: 0px; margin: 0px; padding: 0px;\">MGC premier Human Lentiviral ORF Expression Library \u2013 Technical Manual<\/strong><\/span><\/a><\/p><h5>Product Brochure<\/h5><p style=\"border: 0px; padding: 0px; color: #444444; font-family: Roboto;\"><a style=\"border: 0px; margin: 0px; padding: 0px; transition: all 0.2s ease-in 0s; color: #222222;\" href=\"http:\/\/www.moldiag.in\/wp-content\/uploads\/2016\/06\/MGC-premier-cDNA-and-ORF-brochure.pdf\" target=\"_blank\" rel=\"noopener\"><span style=\"border: 0px; margin: 0px; padding: 0px; color: #1563a1;\"><strong style=\"border: 0px; margin: 0px; padding: 0px;\">MGC\u00a0premier\u00a0cDNA and ORF Clones \u2013 Brochure<\/strong><\/span><\/a><\/p><\/div><\/div>\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-tab-title elementor-tab-mobile-title\" aria-selected=\"false\" data-tab=\"2\" role=\"tab\" tabindex=\"-1\" aria-controls=\"elementor-tab-content-3252\" aria-expanded=\"false\">Design<\/div>\n\t\t\t\t\t<div id=\"elementor-tab-content-3252\" class=\"elementor-tab-content elementor-clearfix\" data-tab=\"2\" role=\"tabpanel\" aria-labelledby=\"elementor-tab-title-3252\" tabindex=\"0\" hidden=\"hidden\"><h2 class=\"yikes-custom-woo-tab-title yikes-custom-woo-tab-title-designs\">Designs<\/h2><h2>Entry ORF and Lentiviral ORF schematics<\/h2><h5><span style=\"font-weight: bold;\">Gateway Cloning<\/span><\/h5><p>Gateway cloning is a recombination-based method for quickly and efficiently creating vectors expressing tagged or native open reading frames (ORFs).<\/p><p><img fetchpriority=\"high\" decoding=\"async\" class=\"wp-image-2264 size-full aligncenter\" src=\"https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/2024\/04\/Gateway-Cloning.png\" alt=\"\" width=\"640\" height=\"480\" srcset=\"https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/2024\/04\/Gateway-Cloning.png 640w, https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/2024\/04\/Gateway-Cloning-200x150.png 200w, https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/2024\/04\/Gateway-Cloning-300x225.png 300w\" sizes=\"(max-width: 640px) 100vw, 640px\" \/><\/p><h5><span style=\"font-weight: bold;\">Lentiviral ORF Expression Vector<\/span><\/h5><p>Schematic of lentiviral ORF expression vector. The lentiviral vector facilitates delivery as plasmid (transfection) or as viral particles (transduction) to dividing or non-dividing cells expanding the number of applications for overexpression.<\/p><p><img decoding=\"async\" class=\"aligncenter wp-image-2265 size-full\" src=\"https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/2024\/04\/Lentiviral-ORF-Expression-Vector.png\" alt=\"\" width=\"640\" height=\"480\" srcset=\"https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/2024\/04\/Lentiviral-ORF-Expression-Vector.png 640w, https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/2024\/04\/Lentiviral-ORF-Expression-Vector-200x150.png 200w, https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/2024\/04\/Lentiviral-ORF-Expression-Vector-300x225.png 300w\" sizes=\"(max-width: 640px) 100vw, 640px\" \/><\/p><\/div>\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-tab-title elementor-tab-mobile-title\" aria-selected=\"false\" data-tab=\"3\" role=\"tab\" tabindex=\"-1\" aria-controls=\"elementor-tab-content-3253\" aria-expanded=\"false\">ORF library<\/div>\n\t\t\t\t\t<div id=\"elementor-tab-content-3253\" class=\"elementor-tab-content elementor-clearfix\" data-tab=\"3\" role=\"tabpanel\" aria-labelledby=\"elementor-tab-title-3253\" tabindex=\"0\" hidden=\"hidden\"><h2><span style=\"text-align: var(--text-align); background-color: var( --e-global-color-c696dce );\">ORF Libraries<\/span><\/h2><p><strong>MGC&nbsp;premier&nbsp;ORF Libraries<\/strong>&nbsp;are genome-scale Gateway-adapted collections developed by the Dana Farber Cancer Institute-Center for Cancer Systems Biology (CCSB Human ORFeome Collection) using the most rigorous sequencing process ensuring the highest sequence quality and confidence.<\/p><ul><li>Complete whole genome collections<\/li><li>Extended ORFeome v8.1 Library available<\/li><li>Lentiviral ORF library \u2013&nbsp;includes over 13,000 ORFs<\/li><li>Available with or without stop codons<\/li><\/ul><h5><strong>MGC&nbsp;premier&nbsp;human ORFeome Collaboration Library<\/strong><\/h5><p>MGC premier human ORFeome Collaboration Library currently represents 9,804 genes with 16,581 clones. The majority of the ORFeome collaboration targets were generated by the Dana Farber Cancer Institute-Center for Cancer Systems Biology (DFCI-CCSB). These full-length, annotated and sequence verified ORFs originate from the existing collection of MGC premier full-length human cDNA clones and have been transferred into Gateway Entry vectors as a ready to use resource for recombinant protein expression. For additional convenience and versatility the ORF clones are available in two formats, with and without stop codons. The ORF clones without stop codons facilitate the synthesis of either C- or N- terminal fusion proteins and clones with stop codons enable the synthesis of native proteins in addition to N-terminal fusion proteins.<\/p><p>The MGC premier ORFeome Collaboration Collection is also available for the mouse genome and represents 1,434 genes with 1,956 clones.<\/p><h5><strong>MGC&nbsp;premier&nbsp;human ORFeome v8.1 Library<\/strong><\/h5><p>MGC premier human ORFeome version 8.1 (hORFeome v8.1) is the newest version of the human ORFeome developed by the Center for Cancer Systems Biology (CCSB). This collection is derived from the Mammalian Gene Collection (MGC) and contains over 12,071 clonally-derived ORFs that represent 11,016 human genes. Each ORF clone in the collection has been verified by next generation sequencing and is provided in Gateway adapted entry vector for fast and convenient transfer to any compatible expression vector.<\/p><h5><strong>MGC&nbsp;premier&nbsp;Human ORFeome Complete Library<\/strong>&nbsp;\u2013&nbsp;ORFeome Collaboration Collection + ORFeome v8.1 Library<\/h5><p>MGC premier human ORFeome Complete Collection is our most extensive entry ORF library and includes both the ORFeome v8.1 and ORFeome Collaboration Collections. This collection provides unique, non-overlapping content and represents 14,918 genes with 22,057 clones.<\/p><h5><strong>MGC&nbsp;premier&nbsp;Lentiviral ORF Expression Library<\/strong><\/h5><p>This human Lentiviral ORF Expression Library was developed through the collaborative efforts of Dana-Farber Cancer Institute, The Broad Institute and the Center for Cancer Systems Biology (CCSB). The collection, which includes over 13,000 ORFs that represent approximately 11,373 genes, provides the most fully sequenced and annotated version of the human ORFeome available. For added convenience the lentiviral ORF expression vector was created to enable expression of a protein of interest with a V5 fusion tag for western blot detection, puri\u00adfication, co-immunoprecipitation, protein localization and FACS analysis.<\/p><\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-e06eaf8 e-flex e-con-boxed e-con e-parent\" data-id=\"e06eaf8\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-e25745e elementor-align-center elementor-widget elementor-widget-button\" data-id=\"e25745e\" data-element_type=\"widget\" data-widget_type=\"button.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-button-wrapper\">\n\t\t\t\t\t<a class=\"elementor-button elementor-button-link elementor-size-sm\" href=\"https:\/\/skyangbio.com\/wordpress\/?page_id=15\">\n\t\t\t\t\t\t<span class=\"elementor-button-content-wrapper\">\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-button-text\">Get A Quote<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>ORF ORF clones Design ORF library ORF clones ORF Clones Gateway-adapted,sequence verified MGC premier ORF Collections provide a comprehensive\u00a0resource of&#8230;<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-1803","page","type-page","status-publish","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v25.9 - 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