{"id":2524,"date":"2024-08-15T01:04:00","date_gmt":"2024-08-15T01:04:00","guid":{"rendered":"https:\/\/skyangbio.com\/wordpress\/?page_id=2524"},"modified":"2025-12-09T21:34:01","modified_gmt":"2025-12-10T03:34:01","slug":"dna-pool-library","status":"publish","type":"page","link":"https:\/\/skyangbio.com\/wordpress\/dna-pool-library\/","title":{"rendered":"DNA pool library"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-page\" data-elementor-id=\"2524\" class=\"elementor elementor-2524\" data-elementor-post-type=\"page\">\n\t\t\t\t<div class=\"elementor-element elementor-element-94762d8 e-flex e-con-boxed e-con e-parent\" data-id=\"94762d8\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-b8b04dd elementor-widget elementor-widget-heading\" data-id=\"b8b04dd\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Skyang Bio Service: POOL AND ARRAYED LIBRARY<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-7822bad e-flex e-con-boxed e-con e-parent\" data-id=\"7822bad\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-0d48d3f elementor-widget elementor-widget-image\" data-id=\"0d48d3f\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"1024\" height=\"576\" src=\"https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/2024\/12\/library-promotion-1024x576.jpg\" class=\"attachment-large size-large wp-image-304546\" alt=\"\" srcset=\"https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/2024\/12\/library-promotion-1024x576.jpg 1024w, https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/2024\/12\/library-promotion-e1734472490861-200x113.jpg 200w, https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/2024\/12\/library-promotion-300x169.jpg 300w, https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/2024\/12\/library-promotion-768x432.jpg 768w, https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/2024\/12\/library-promotion-e1734472490861.jpg 400w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-41452e70 elementor-widget elementor-widget-text-editor\" data-id=\"41452e70\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>\u00a0<\/p><p class=\"x_MsoNormal\" data-olk-copy-source=\"MessageBody\">Pooled CRISPR screens are only as effective as the quality of the libraries used. At\u00a0<b>Skyang Bio<\/b>, we specialize in creating custom CRISPR libraries that are tailored to your specific research needs. Whether you&#8217;re looking to perform CRISPR knockout (CRISPRko), activation (CRISPRa), or interference (CRISPRi) screens, we offer cutting-edge solutions that support your experimental goals with precision and efficiency.<\/p><p class=\"x_MsoNormal\">Our expertly designed CRISPR pooled and arrayed libraries ensure reproducibility and high screening quality for a wide range of applications.<\/p><p class=\"x_MsoNormal\"><b>Ready to take your research to the next level?<\/b>\u00a0<a href=\"https:\/\/skyangbio.com\/wordpress\/?page_id=15\" target=\"_blank\" rel=\"noopener\">Contact\u00a0<b>Skyang Bio<\/b><\/a>\u00a0today to discuss how we can help you achieve your project\u2019s goals with our custom CRISPR technologies.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-3e85ebfe elementor-mobile-align-justify elementor-widget-mobile__width-inherit elementor-align-right elementor-widget elementor-widget-button\" data-id=\"3e85ebfe\" data-element_type=\"widget\" data-widget_type=\"button.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-button-wrapper\">\n\t\t\t\t\t<a class=\"elementor-button elementor-button-link elementor-size-sm\" href=\"https:\/\/skyangbio.com\/wordpress\/?page_id=15\">\n\t\t\t\t\t\t<span class=\"elementor-button-content-wrapper\">\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-button-text\">Get A Quote<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-39c8a44 e-flex e-con-boxed e-con e-parent\" data-id=\"39c8a44\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-68efa22 elementor-widget elementor-widget-heading\" data-id=\"68efa22\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Highlights<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-1fca443 e-flex e-con-boxed e-con e-parent\" data-id=\"1fca443\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-5fcbbbe elementor-widget elementor-widget-heading\" data-id=\"5fcbbbe\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Customer Screening pools<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-15ef378 elementor-widget elementor-widget-text-editor\" data-id=\"15ef378\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Customers can use any of our CRISPR or shRNA lentiviral vectors for creating custom screening pools.<\/p><ul><li>Client identifies genes of interest to be targeted (i.e. druggable genes) and desired vector<\/li><li>Skyang uses its gRNA algorithm or shRNA algorithm to design targeting gRNAs or shRNA<\/li><li>Skyang synthesizes gRNAs or shRNAs and clones into vector backbone<\/li><li>The screening pool is qualified by Next-Gen Sequencing to ensure equal representation of every construct.<\/li><li>Skyang packages the screening pool into viral particles and performs functional titer analysis.<img decoding=\"async\" class=\"alignnone size-full wp-image-304566\" src=\"https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/2024\/12\/Pool-procedure.png\" alt=\"\" width=\"4039\" height=\"801\" srcset=\"https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/2024\/12\/Pool-procedure.png 4039w, https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/2024\/12\/Pool-procedure-200x40.png 200w, https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/2024\/12\/Pool-procedure-300x59.png 300w, https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/2024\/12\/Pool-procedure-1024x203.png 1024w, https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/2024\/12\/Pool-procedure-768x152.png 768w, https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/2024\/12\/Pool-procedure-1536x305.png 1536w, https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/2024\/12\/Pool-procedure-2048x406.png 2048w\" sizes=\"(max-width: 4039px) 100vw, 4039px\" \/><\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-1c165f4 e-flex e-con-boxed e-con e-parent\" data-id=\"1c165f4\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-3a7fa01 elementor-widget elementor-widget-heading\" data-id=\"3a7fa01\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Customer Screening Pools QC<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-e17e06a elementor-widget elementor-widget-text-editor\" data-id=\"e17e06a\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Skyang Bio offers a Certificate of Analysis for all screening pools, showing distribution of gRNAs\/shRNAs as determined by Nex\u2013Gen Sequencing.\u00a0<\/p><p><img decoding=\"async\" class=\"alignnone wp-image-306559 size-large\" src=\"https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/COA-representation2-1024x841.png\" alt=\"\" width=\"1024\" height=\"841\" srcset=\"https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/COA-representation2-1024x841.png 1024w, https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/COA-representation2-300x246.png 300w, https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/COA-representation2-768x631.png 768w, https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/COA-representation2-200x164.png 200w, https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/COA-representation2.png 1104w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-7cd8dab elementor-widget elementor-widget-text-editor\" data-id=\"7cd8dab\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Lentiviral pools will be evaluated by functional viral titer*.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-9f30aae elementor-widget elementor-widget-text-editor\" data-id=\"9f30aae\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-304577\" src=\"https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/2024\/12\/LT.png\" alt=\"\" width=\"446\" height=\"340\" srcset=\"https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/2024\/12\/LT.png 446w, https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/2024\/12\/LT-200x152.png 200w, https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/2024\/12\/LT-300x229.png 300w\" sizes=\"(max-width: 446px) 100vw, 446px\" \/><\/p><p>*Skyang Bio performs a <b>functional titer <\/b>on all lentiviral particles delivered. Functional titer analysis (opposed to physical titer analysis) is a more accurate measurement for calculating MOIs that are used in screening experiments.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-487b3f1 e-flex e-con-boxed e-con e-parent\" data-id=\"487b3f1\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-ff25935 elementor-widget elementor-widget-heading\" data-id=\"ff25935\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Customer Arrayed Library<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-e7daab5 elementor-widget elementor-widget-text-editor\" data-id=\"e7daab5\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Streamlined work-flows allow Skyang to be one of the few vendors that offers customized arrayed CRISPR and shRNA libraries in 96-well format<img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-304593\" src=\"https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/2024\/12\/Arrayed-lib.png\" alt=\"\" width=\"1740\" height=\"656\" srcset=\"https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/2024\/12\/Arrayed-lib.png 1740w, https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/2024\/12\/Arrayed-lib-200x75.png 200w, https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/2024\/12\/Arrayed-lib-300x113.png 300w, https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/2024\/12\/Arrayed-lib-1024x386.png 1024w, https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/2024\/12\/Arrayed-lib-768x290.png 768w, https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/2024\/12\/Arrayed-lib-1536x579.png 1536w\" sizes=\"(max-width: 1740px) 100vw, 1740px\" \/><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-7cf4142 elementor-widget elementor-widget-heading\" data-id=\"7cf4142\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Please contact us for more information and quote.<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-c2c3b31 e-flex e-con-boxed e-con e-parent\" data-id=\"c2c3b31\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-12cfa08 elementor-widget elementor-widget-wpforms\" data-id=\"12cfa08\" data-element_type=\"widget\" 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