{"id":2654,"date":"2024-08-15T00:56:34","date_gmt":"2024-08-15T00:56:34","guid":{"rendered":"https:\/\/skyangbio.com\/wordpress\/?page_id=2654"},"modified":"2024-08-18T22:12:08","modified_gmt":"2024-08-18T22:12:08","slug":"cloning-on-demand","status":"publish","type":"page","link":"https:\/\/skyangbio.com\/wordpress\/cloning-on-demand\/","title":{"rendered":"Cloning on demand"},"content":{"rendered":"\t\t
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Services<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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Cloning Services: Cloning on Demand<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\tGet A Quote<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Skyang Biotech\u2019s Cloning on Demand service based on our hundreds optimized vectors, all validated through rigorous testing, is designed to meet your research needs with unmatched precision and efficiency.\u00a0<\/p>

Our Cloning on Demand services include sgRNA gene set, shRNA gene set and ORF cloning.<\/p>

What sets our service apart is the ease of use and cost-effectiveness. Our online vector select system allows for esily pick the correct vecotrs for your project, minimizing the need for de novo gene synthesis. This, coupled with our unbeatable pricing and rapid turnaround times, makes Skyang Biotech\u2019s cloning service a smarter choice than traditional DIY methods.<\/p>

Additionally, we offer related services such as plasmid DNA preparation and lenti virus packaging, ensuring you have all the tools you need for successful gene modulation. With a commitment to quality and reliability, Skyang Biotech is your trusted partner for vector cloning and beyond.<\/p>

  • \u00a0Target gRNA gene sets for gene knockout (CRISPR) gene activation (CRISPRa) and gene inhibition (CRISPRi)<\/li>
  • \u00a0Includes 3 gRNA constructs plus a non-targeting control<\/li>
  • Target shRNA gene sets<\/li>
  • Includes 3 shRNA constructs plus a non-targeting control<\/li>
  • Full-length cDNAs and ORFs<\/li>
  • Mutated genes for rescue experiments<\/li><\/ul>

    \n\n\n\n\t\n\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t
    COD Type<\/strong><\/th>Vector category<\/strong><\/th>Content<\/strong><\/th>Format<\/strong><\/th>Price<\/strong><\/th>Turnaround<\/strong><\/th><\/th>\n<\/tr>\n<\/thead>\n
    target gene set (sgRNA)<\/td>pCLIP-gRNA<\/td>3 top ranking sgRNAs\u00a0per gene with non-targeting negative control in selected pCLIP-gRNA vector (4 vectors)<\/td>Glycerol stock<\/td>775<\/td>2-3 weeks<\/td><\/td>\n<\/tr>\n
    Virus (100ul, 10^7)<\/td>2315<\/td>4-5 weeks<\/td><\/td>\n<\/tr>\n
    Virus (100ul, 10^8)<\/td>3295<\/td>4-5 weeks<\/td><\/td>\n<\/tr>\n
    target gene set (sgRNA)<\/td>pCLIP-ALL<\/td>3 top ranking sgRNAs\u00a0per gene with non-targeting negative control in selected pCLIP-all vector (4 vectors)<\/td>Glycerol stock<\/td>775<\/td>2-3 weeks<\/td><\/td>\n<\/tr>\n
    Virus (100ul, 10^6)<\/td>3245<\/td>4-5 weeks<\/td><\/td>\n<\/tr>\n
    target gene set (sgRNA)<\/td>dual gRNA<\/td>6 top ranking sgRNAs\u00a0per gene with non-targeting negative control in selected pCLIP-dual vector (4 vectors)<\/td>Glycerol stock<\/td>775<\/td>2-3 weeks<\/td><\/td>\n<\/tr>\n
    Virus (100ul, 10^7)<\/td>3295<\/td>4-5 weeks<\/td><\/td>\n<\/tr>\n
    target gene set (shRNA)<\/td>Constitutive<\/td>3 top ranking shRNAs\u00a0per gene with non-targeting negative control in selected pZIP vector (4 vectors)<\/td>Glycerol stock<\/td>775<\/td>2-3 weeks<\/td><\/td>\n<\/tr>\n
    Virus (100ul, 10^7)<\/td>2315<\/td>4-5 weeks<\/td><\/td>\n<\/tr>\n
    Virus (100ul, 10^8)<\/td>3295<\/td>4-5 weeks<\/td><\/td>\n<\/tr>\n
    target gene set (shRNA)<\/td>Inducible<\/td>3 top ranking shRNAs\u00a0per gene with non-targeting negative control in selected pZIP vector (4 vectors)<\/td>Glycerol stock<\/td>775<\/td>2-3 weeks<\/td><\/td>\n<\/tr>\n
    Virus (100ul, 10^7)<\/td>3295<\/td>4-5 weeks<\/td><\/td>\n<\/tr>\n
    Gene Expression (< 1.7 kb)<\/td>Constitutive\/Inducible<\/td>Targeting ORF in selected LentiORF vector (1 vector)<\/td>Glycerol stock<\/td>579<\/td>2-3 weeks<\/td><\/td>\n<\/tr>\n
    Virus (100ul, concentrated)<\/td>1479<\/td>4-5 weeks<\/td><\/td>\n<\/tr>\n
    Gene Expression (1.7 – 3.5 kb)<\/td>Constitutive\/Inducible<\/td>Targeting ORF in selected LentiORF vector (1 vector)<\/td>Glycerol stock<\/td>879<\/td>3-4 weeks<\/td><\/td>\n<\/tr>\n
    Virus (100ul, concentrated)<\/td>1779<\/td>4-6 weeks<\/td><\/td>\n<\/tr>\n
    Gene Expression (3.5-5 kb)<\/td>Constitutive\/Inducible<\/td>Targeting ORF in selected LentiORF vector (1 vector)<\/td>Glycerol stock<\/td>1579<\/td>3-4 weeks<\/td><\/td>\n<\/tr>\n
    Virus (100ul, concentrated)<\/td>2539<\/td>4-6 weeks<\/td><\/td>\n<\/tr>\n
    Gene Expression (>5 kb)<\/td>Constitutive\/Inducible<\/td>Targeting ORF in selected LentiORF vector (1 vector)<\/td>Glycerol stock<\/td>Quote<\/td>Variable<\/td><\/td>\n<\/tr>\n
    Virus (100ul, concentrated)<\/td>Quote<\/td>Variable<\/td><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"

    Services Cloning Services: Cloning on Demand Get A Quote Skyang Biotech\u2019s Cloning on Demand service based on our hundreds optimized…<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-2654","page","type-page","status-publish","hentry"],"yoast_head":"\nCloning on demand - Skyang Bio<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/skyangbio.com\/wordpress\/cloning-on-demand\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Cloning on demand - Skyang Bio\" \/>\n<meta property=\"og:description\" content=\"Services Cloning Services: Cloning on Demand Get A Quote Skyang Biotech\u2019s Cloning on Demand service based on our hundreds optimized...\" \/>\n<meta property=\"og:url\" content=\"https:\/\/skyangbio.com\/wordpress\/cloning-on-demand\/\" \/>\n<meta property=\"og:site_name\" content=\"Skyang Bio\" \/>\n<meta property=\"article:modified_time\" content=\"2024-08-18T22:12:08+00:00\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/skyangbio.com\/wordpress\/cloning-on-demand\/\",\"url\":\"https:\/\/skyangbio.com\/wordpress\/cloning-on-demand\/\",\"name\":\"Cloning on demand - 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