{"id":304884,"date":"2024-12-21T18:29:13","date_gmt":"2024-12-21T18:29:13","guid":{"rendered":"https:\/\/skyangbio.com\/wordpress\/?page_id=304884"},"modified":"2024-12-21T18:44:31","modified_gmt":"2024-12-21T18:44:31","slug":"shrna-mouse-whole-genome-arrayed-library","status":"publish","type":"page","link":"https:\/\/skyangbio.com\/wordpress\/shrna-mouse-whole-genome-arrayed-library\/","title":{"rendered":"shRNA Mouse Whole Genome Arrayed Library"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-page\" data-elementor-id=\"304884\" class=\"elementor elementor-304884\" data-elementor-post-type=\"page\">\n\t\t\t\t<div class=\"elementor-element elementor-element-e5468ae e-flex e-con-boxed e-con e-parent\" data-id=\"e5468ae\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-5359961 elementor-widget elementor-widget-heading\" data-id=\"5359961\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">shRNA Mouse Whole Genome Arrayed Library<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-9f4166e e-flex e-con-boxed e-con e-parent\" data-id=\"9f4166e\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-0d732c6 elementor-widget elementor-widget-text-editor\" data-id=\"0d732c6\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"vc_row wpb_row vc_inner vc_row-fluid\"><div class=\"wpb_column vc_column_container vc_col-sm-8\"><div class=\"vc_column-inner\"><div class=\"wpb_wrapper\"><div class=\"wpb_text_column wpb_content_element\"><div class=\"wpb_wrapper\"><h6>shERWOOD shRNA design + Optimized shRNA processing = Superior Knockdown<\/h6><p>SkyangBio\u2019s shERWOOD-UltramiR\u2122 shRNA libraries are next-generation, vector-based RNAi triggers designed using the proprietary shERWOOD algorithm that was developed and validated in Dr. Gregory Hannon\u2019s laboratory at Cold Spring Harbor Laboratory (Knott et al 2014). The library uses an alternate microRNA scaffold called \u201cUltramiR\u201c. The UltramiR scaffold has been optimized for increased shRNA processing and potency based on the key determinants for primary microRNA processing (Auyeung et al 2013).<\/p><p>The shERWOOD algorithm is based on the functional testing of over 250,000 shRNA sequences using a high-throughput sensor assay and uses key sequence characteristics for predicting shRNA potency to select the rare shRNA designs that are potent at single copy representation in the genome.<\/p><h3>Features<\/h3><ul><li>Over <strong>18,000<\/strong> mouse genes targeted<\/li><li>Each human gene is targeted by an average of <strong>5<\/strong> shRNA constructs<\/li><li>The shRNAs are cloned into a retroviral vector that contains a Neomycin selection marker and a ZsGreen fluorescent reporter<\/li><\/ul><\/div><\/div><\/div><\/div><\/div><div class=\"wpb_column vc_column_container vc_col-sm-4\"><div class=\"vc_column-inner\"><div class=\"wpb_wrapper\"><div class=\"wpb_single_image wpb_content_element vc_align_left wpb_content_element\"><figure class=\"wpb_wrapper vc_figure\"><div class=\"vc_single_image-wrapper vc_box_border_grey\"><img fetchpriority=\"high\" decoding=\"async\" class=\"vc_single_image-img attachment-full lazyloaded\" title=\"shERWOOD-Algorithm-Screen\" src=\"https:\/\/www.stratech.co.uk\/wp-content\/uploads\/2023\/07\/shERWOOD-Algorithm-Screen.jpg\" sizes=\"(max-width: 300px) 100vw, 300px\" srcset=\"https:\/\/www.stratech.co.uk\/wp-content\/uploads\/2023\/07\/shERWOOD-Algorithm-Screen.jpg 300w, https:\/\/www.stratech.co.uk\/wp-content\/uploads\/2023\/07\/shERWOOD-Algorithm-Screen-150x150.jpg 150w\" alt=\"\" width=\"300\" height=\"300\" data-src=\"https:\/\/www.stratech.co.uk\/wp-content\/uploads\/2023\/07\/shERWOOD-Algorithm-Screen.jpg\" data-srcset=\"https:\/\/www.stratech.co.uk\/wp-content\/uploads\/2023\/07\/shERWOOD-Algorithm-Screen.jpg 300w, https:\/\/www.stratech.co.uk\/wp-content\/uploads\/2023\/07\/shERWOOD-Algorithm-Screen-150x150.jpg 150w\" data-sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/div><\/figure><\/div><div class=\"vc_btn3-container vc_btn3-center vc_do_btn\"><a class=\"vc_general vc_btn3 vc_btn3-size-md vc_btn3-shape-rounded vc_btn3-style-custom vc_btn3-block\" title=\"\" href=\"https:\/\/www.cell.com\/molecular-cell\/fulltext\/S1097-2765(14)00835-1\" target=\"_blank\" rel=\"noopener\">Access Publication<\/a><\/div><\/div><\/div><\/div><\/div><div class=\"vc_row wpb_row vc_inner vc_row-fluid\"><div class=\"wpb_column vc_column_container vc_col-sm-12\"><div class=\"vc_column-inner\"><div class=\"wpb_wrapper\"><div class=\"wpb_single_image wpb_content_element vc_align_center wpb_content_element\"><figure class=\"wpb_wrapper vc_figure\"><div class=\"vc_single_image-wrapper vc_box_border_grey\"><img decoding=\"async\" class=\"vc_single_image-img attachment-full lazyloaded\" title=\"pLMN-vector-schematic\" src=\"https:\/\/www.stratech.co.uk\/wp-content\/uploads\/2023\/07\/pLMN-vector-schematic.png\" sizes=\"(max-width: 700px) 100vw, 700px\" srcset=\"https:\/\/www.stratech.co.uk\/wp-content\/uploads\/2023\/07\/pLMN-vector-schematic.png 700w, https:\/\/www.stratech.co.uk\/wp-content\/uploads\/2023\/07\/pLMN-vector-schematic-300x27.png 300w\" alt=\"\" width=\"700\" height=\"64\" data-src=\"https:\/\/www.stratech.co.uk\/wp-content\/uploads\/2023\/07\/pLMN-vector-schematic.png\" data-srcset=\"https:\/\/www.stratech.co.uk\/wp-content\/uploads\/2023\/07\/pLMN-vector-schematic.png 700w, https:\/\/www.stratech.co.uk\/wp-content\/uploads\/2023\/07\/pLMN-vector-schematic-300x27.png 300w\" data-sizes=\"(max-width: 700px) 100vw, 700px\" \/><\/div><\/figure><\/div><div class=\"wpb_text_column wpb_content_element\"><div class=\"wpb_wrapper\"><h3>Advantages<\/h3><ul><li>The proprietary shERWOOD algorithm was used to design all shRNA. Read the publication in Molecular Cell<\/li><li>The UltramiR microRNA scaffold increases shRNA processing and potency<\/li><li>All mouse shRNA library constructs are 100% sequence-confirmed<\/li><li>Glycerol stock 96-well plates are easily maintained and offer a convenient workflow<\/li><\/ul><h3>Deliverables<\/h3><ul><li>Human shRNA library: <strong>898<\/strong> 96-well glycerol stock plates with barcoded labels<\/li><li>Data file for 96-well plate clone mapping<\/li><\/ul><\/div><\/div><\/div><\/div><\/div><\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-0bddb31 e-flex e-con-boxed e-con e-parent\" data-id=\"0bddb31\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-b5e1b28 elementor-align-center elementor-widget elementor-widget-button\" data-id=\"b5e1b28\" data-element_type=\"widget\" data-widget_type=\"button.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-button-wrapper\">\n\t\t\t\t\t<a class=\"elementor-button elementor-button-link elementor-size-sm\" href=\"https:\/\/skyangbio.com\/wordpress\/?page_id=15\" target=\"_blank\">\n\t\t\t\t\t\t<span class=\"elementor-button-content-wrapper\">\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-button-text\">Get A Quote<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>shRNA Mouse Whole Genome Arrayed Library shERWOOD shRNA design + Optimized shRNA processing = Superior Knockdown SkyangBio\u2019s shERWOOD-UltramiR\u2122 shRNA libraries&#8230;<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-304884","page","type-page","status-publish","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v25.9 - 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