{"id":304911,"date":"2024-12-21T18:48:02","date_gmt":"2024-12-21T18:48:02","guid":{"rendered":"https:\/\/skyangbio.com\/wordpress\/?page_id=304911"},"modified":"2024-12-21T18:51:03","modified_gmt":"2024-12-21T18:51:03","slug":"crispr-dual-grna-whole-genome-arrayed-library","status":"publish","type":"page","link":"https:\/\/skyangbio.com\/wordpress\/crispr-dual-grna-whole-genome-arrayed-library\/","title":{"rendered":"CRISPR Dual gRNA Whole Genome Arrayed Library"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-page\" data-elementor-id=\"304911\" class=\"elementor elementor-304911\" data-elementor-post-type=\"page\">\n\t\t\t\t<div class=\"elementor-element elementor-element-cbf7f8a e-flex e-con-boxed e-con e-parent\" data-id=\"cbf7f8a\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-2f8409c elementor-widget elementor-widget-heading\" data-id=\"2f8409c\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">CRISPR Dual gRNA Whole Genome Arrayed Library-Human<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-eac7cf8 e-flex e-con-boxed e-con e-parent\" data-id=\"eac7cf8\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-a72ed36 elementor-widget elementor-widget-text-editor\" data-id=\"a72ed36\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"vc_row wpb_row vc_inner vc_row-fluid\"><div class=\"wpb_column vc_column_container vc_col-sm-8\"><div class=\"vc_column-inner\"><div class=\"wpb_wrapper\"><div class=\"wpb_text_column wpb_content_element\"><div class=\"wpb_wrapper\"><div class=\"vc_row wpb_row vc_inner vc_row-fluid\"><div class=\"wpb_column vc_column_container vc_col-sm-8\"><div class=\"vc_column-inner\"><div class=\"wpb_wrapper\"><div class=\"wpb_text_column wpb_content_element\"><div class=\"wpb_wrapper\"><h6>Dual gRNA lentiviral vector developed using the CRoatan gRNA design algorithm for superior knockout efficiency<\/h6><p>SkyangBio\u2019s dual gRNA CRISPR library was developed in collaboration with Cold Spring Harbor Laboratory. The library targets over 18,000 human genes and is the only dual gRNA arrayed library in the industry. The CRISPR library is conveniently delivered as glycerol stock clones in 96-well plate format.<\/p><h3>Features<\/h3><ul><li>Over 18,000 human genes targeted<\/li><li>Each human gene is targeted by 3-5 unique dual gRNA constructs<\/li><li>Every dual gRNA construct has been 100% sequence-verified<\/li><li>Over 1,000 negative controls are contained in the arrayed library<\/li><li>Each dual gRNA construct contains a unique barcode, a puromycin selection marker and a GFP fluorescent reporter<\/li><\/ul><\/div><\/div><\/div><\/div><\/div><div class=\"wpb_column vc_column_container vc_col-sm-4\"><div class=\"vc_column-inner\"><div class=\"wpb_wrapper\"><div class=\"wpb_single_image wpb_content_element vc_align_left wpb_content_element\"><figure class=\"wpb_wrapper vc_figure\"><div class=\"vc_single_image-wrapper   vc_box_border_grey\"><img fetchpriority=\"high\" decoding=\"async\" class=\"vc_single_image-img attachment-full lazyloaded\" title=\"sgRNA-selection-algorithm-light\" src=\"https:\/\/www.stratech.co.uk\/wp-content\/uploads\/2023\/07\/sgRNA-selection-algorithm-light.png\" alt=\"\" width=\"300\" height=\"232\" data-src=\"https:\/\/www.stratech.co.uk\/wp-content\/uploads\/2023\/07\/sgRNA-selection-algorithm-light.png\" \/><\/div><\/figure><\/div><div class=\"vc_btn3-container vc_btn3-center vc_do_btn\"><a class=\"vc_general vc_btn3 vc_btn3-size-md vc_btn3-shape-rounded vc_btn3-style-custom vc_btn3-block\" title=\"\" href=\"https:\/\/www.cell.com\/molecular-cell\/fulltext\/S1097-2765(17)30464-1\" target=\"_blank\" rel=\"noopener\">Access Publication<\/a><\/div><\/div><\/div><\/div><\/div><div class=\"vc_row wpb_row vc_inner vc_row-fluid\"><div class=\"wpb_column vc_column_container vc_col-sm-12\"><div class=\"vc_column-inner\"><div class=\"wpb_wrapper\"><div class=\"wpb_single_image wpb_content_element vc_align_left wpb_content_element\"><figure class=\"wpb_wrapper vc_figure\"><a class=\"vc_single_image-wrapper   vc_box_border_grey\" href=\"https:\/\/www.stratech.co.uk\/wp-content\/uploads\/2023\/07\/CRISPR_pCLIP-dual-gRNA_vector-schematic-Whole-Genome-Library.png\" target=\"_blank\" rel=\"noopener\"><img decoding=\"async\" class=\"vc_single_image-img attachment-full lazyloaded\" title=\"CRISPR_pCLIP-dual-gRNA_vector-schematic-Whole-Genome-Library\" src=\"https:\/\/www.stratech.co.uk\/wp-content\/uploads\/2023\/07\/CRISPR_pCLIP-dual-gRNA_vector-schematic-Whole-Genome-Library.png\" sizes=\"(max-width: 850px) 100vw, 850px\" srcset=\"https:\/\/www.stratech.co.uk\/wp-content\/uploads\/2023\/07\/CRISPR_pCLIP-dual-gRNA_vector-schematic-Whole-Genome-Library.png 850w, https:\/\/www.stratech.co.uk\/wp-content\/uploads\/2023\/07\/CRISPR_pCLIP-dual-gRNA_vector-schematic-Whole-Genome-Library-300x23.png 300w, https:\/\/www.stratech.co.uk\/wp-content\/uploads\/2023\/07\/CRISPR_pCLIP-dual-gRNA_vector-schematic-Whole-Genome-Library-768x59.png 768w\" alt=\"\" width=\"850\" height=\"65\" data-src=\"https:\/\/www.stratech.co.uk\/wp-content\/uploads\/2023\/07\/CRISPR_pCLIP-dual-gRNA_vector-schematic-Whole-Genome-Library.png\" data-srcset=\"https:\/\/www.stratech.co.uk\/wp-content\/uploads\/2023\/07\/CRISPR_pCLIP-dual-gRNA_vector-schematic-Whole-Genome-Library.png 850w, https:\/\/www.stratech.co.uk\/wp-content\/uploads\/2023\/07\/CRISPR_pCLIP-dual-gRNA_vector-schematic-Whole-Genome-Library-300x23.png 300w, https:\/\/www.stratech.co.uk\/wp-content\/uploads\/2023\/07\/CRISPR_pCLIP-dual-gRNA_vector-schematic-Whole-Genome-Library-768x59.png 768w\" data-sizes=\"(max-width: 850px) 100vw, 850px\" \/><\/a><\/figure><\/div><div class=\"wpb_text_column wpb_content_element\"><div class=\"wpb_wrapper\"><h3>Advantages<\/h3><ul><li>The Croatan algorithm was used to design all gRNAs. Read the publication in Molecular Cell<\/li><li>The dual gRNA design increases the likelihood of CRISPR Cas9 cuts and frameshifts for efficient gene knockout<\/li><li>Lentiviral vector packages efficiently for high-titer viral particles<\/li><li>All CRISPR library constructs are 100% sequence-confirmed<\/li><li>Glycerol stock 96-well plates are easily maintained and offer convenient work-flow<\/li><\/ul><h3>Deliverable<\/h3><ul><li>670 96-well glycerol stock plates with barcoded labels<\/li><li>100% sequence-confirmed dual gRNA constructs<\/li><li>Datafile for 96-well plate clone mapping<\/li><\/ul><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-05c2c0b e-flex e-con-boxed e-con e-parent\" data-id=\"05c2c0b\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-5564aed elementor-align-center elementor-widget elementor-widget-button\" data-id=\"5564aed\" data-element_type=\"widget\" data-widget_type=\"button.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-button-wrapper\">\n\t\t\t\t\t<a class=\"elementor-button elementor-button-link elementor-size-sm\" href=\"https:\/\/skyangbio.com\/wordpress\/?page_id=15\" target=\"_blank\">\n\t\t\t\t\t\t<span class=\"elementor-button-content-wrapper\">\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-button-text\">Get A Quote<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>CRISPR Dual gRNA Whole Genome Arrayed Library-Human Dual gRNA lentiviral vector developed using the CRoatan gRNA design algorithm for superior&#8230;<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-304911","page","type-page","status-publish","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v25.9 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>CRISPR Dual gRNA Whole Genome Arrayed Library - Skyang Bio<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/skyangbio.com\/wordpress\/crispr-dual-grna-whole-genome-arrayed-library\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"CRISPR Dual gRNA Whole Genome Arrayed Library - Skyang Bio\" \/>\n<meta property=\"og:description\" content=\"CRISPR Dual gRNA Whole Genome Arrayed Library-Human Dual gRNA lentiviral vector developed using the CRoatan gRNA design algorithm for superior...\" \/>\n<meta property=\"og:url\" content=\"https:\/\/skyangbio.com\/wordpress\/crispr-dual-grna-whole-genome-arrayed-library\/\" \/>\n<meta property=\"og:site_name\" content=\"Skyang Bio\" \/>\n<meta property=\"article:modified_time\" content=\"2024-12-21T18:51:03+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.stratech.co.uk\/wp-content\/uploads\/2023\/07\/sgRNA-selection-algorithm-light.png\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/skyangbio.com\/wordpress\/crispr-dual-grna-whole-genome-arrayed-library\/\",\"url\":\"https:\/\/skyangbio.com\/wordpress\/crispr-dual-grna-whole-genome-arrayed-library\/\",\"name\":\"CRISPR Dual gRNA Whole Genome Arrayed Library - Skyang Bio\",\"isPartOf\":{\"@id\":\"https:\/\/skyangbio.com\/wordpress\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\/\/skyangbio.com\/wordpress\/crispr-dual-grna-whole-genome-arrayed-library\/#primaryimage\"},\"image\":{\"@id\":\"https:\/\/skyangbio.com\/wordpress\/crispr-dual-grna-whole-genome-arrayed-library\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/www.stratech.co.uk\/wp-content\/uploads\/2023\/07\/sgRNA-selection-algorithm-light.png\",\"datePublished\":\"2024-12-21T18:48:02+00:00\",\"dateModified\":\"2024-12-21T18:51:03+00:00\",\"breadcrumb\":{\"@id\":\"https:\/\/skyangbio.com\/wordpress\/crispr-dual-grna-whole-genome-arrayed-library\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/skyangbio.com\/wordpress\/crispr-dual-grna-whole-genome-arrayed-library\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/skyangbio.com\/wordpress\/crispr-dual-grna-whole-genome-arrayed-library\/#primaryimage\",\"url\":\"https:\/\/www.stratech.co.uk\/wp-content\/uploads\/2023\/07\/sgRNA-selection-algorithm-light.png\",\"contentUrl\":\"https:\/\/www.stratech.co.uk\/wp-content\/uploads\/2023\/07\/sgRNA-selection-algorithm-light.png\"},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/skyangbio.com\/wordpress\/crispr-dual-grna-whole-genome-arrayed-library\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\/\/skyangbio.com\/wordpress\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"CRISPR Dual gRNA Whole Genome Arrayed Library\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/skyangbio.com\/wordpress\/#website\",\"url\":\"https:\/\/skyangbio.com\/wordpress\/\",\"name\":\"Skyang Bio\",\"description\":\"Gene Expression, RNAi and CRISPR Genomic Tools and Services\",\"publisher\":{\"@id\":\"https:\/\/skyangbio.com\/wordpress\/#organization\"},\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/skyangbio.com\/wordpress\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"},{\"@type\":\"Organization\",\"@id\":\"https:\/\/skyangbio.com\/wordpress\/#organization\",\"name\":\"Skyang Bio\",\"url\":\"https:\/\/skyangbio.com\/wordpress\/\",\"logo\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/skyangbio.com\/wordpress\/#\/schema\/logo\/image\/\",\"url\":\"https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/cropped-Skyang-Bio_Logo_New.png\",\"contentUrl\":\"https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/cropped-Skyang-Bio_Logo_New.png\",\"width\":364,\"height\":283,\"caption\":\"Skyang Bio\"},\"image\":{\"@id\":\"https:\/\/skyangbio.com\/wordpress\/#\/schema\/logo\/image\/\"}}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"CRISPR Dual gRNA Whole Genome Arrayed Library - Skyang Bio","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/skyangbio.com\/wordpress\/crispr-dual-grna-whole-genome-arrayed-library\/","og_locale":"en_US","og_type":"article","og_title":"CRISPR Dual gRNA Whole Genome Arrayed Library - Skyang Bio","og_description":"CRISPR Dual gRNA Whole Genome Arrayed Library-Human Dual gRNA lentiviral vector developed using the CRoatan gRNA design algorithm for superior...","og_url":"https:\/\/skyangbio.com\/wordpress\/crispr-dual-grna-whole-genome-arrayed-library\/","og_site_name":"Skyang Bio","article_modified_time":"2024-12-21T18:51:03+00:00","og_image":[{"url":"https:\/\/www.stratech.co.uk\/wp-content\/uploads\/2023\/07\/sgRNA-selection-algorithm-light.png","type":"","width":"","height":""}],"twitter_card":"summary_large_image","twitter_misc":{"Est. reading time":"1 minute"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/skyangbio.com\/wordpress\/crispr-dual-grna-whole-genome-arrayed-library\/","url":"https:\/\/skyangbio.com\/wordpress\/crispr-dual-grna-whole-genome-arrayed-library\/","name":"CRISPR Dual gRNA Whole Genome Arrayed Library - Skyang Bio","isPartOf":{"@id":"https:\/\/skyangbio.com\/wordpress\/#website"},"primaryImageOfPage":{"@id":"https:\/\/skyangbio.com\/wordpress\/crispr-dual-grna-whole-genome-arrayed-library\/#primaryimage"},"image":{"@id":"https:\/\/skyangbio.com\/wordpress\/crispr-dual-grna-whole-genome-arrayed-library\/#primaryimage"},"thumbnailUrl":"https:\/\/www.stratech.co.uk\/wp-content\/uploads\/2023\/07\/sgRNA-selection-algorithm-light.png","datePublished":"2024-12-21T18:48:02+00:00","dateModified":"2024-12-21T18:51:03+00:00","breadcrumb":{"@id":"https:\/\/skyangbio.com\/wordpress\/crispr-dual-grna-whole-genome-arrayed-library\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/skyangbio.com\/wordpress\/crispr-dual-grna-whole-genome-arrayed-library\/"]}]},{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/skyangbio.com\/wordpress\/crispr-dual-grna-whole-genome-arrayed-library\/#primaryimage","url":"https:\/\/www.stratech.co.uk\/wp-content\/uploads\/2023\/07\/sgRNA-selection-algorithm-light.png","contentUrl":"https:\/\/www.stratech.co.uk\/wp-content\/uploads\/2023\/07\/sgRNA-selection-algorithm-light.png"},{"@type":"BreadcrumbList","@id":"https:\/\/skyangbio.com\/wordpress\/crispr-dual-grna-whole-genome-arrayed-library\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/skyangbio.com\/wordpress\/"},{"@type":"ListItem","position":2,"name":"CRISPR Dual gRNA Whole Genome Arrayed Library"}]},{"@type":"WebSite","@id":"https:\/\/skyangbio.com\/wordpress\/#website","url":"https:\/\/skyangbio.com\/wordpress\/","name":"Skyang Bio","description":"Gene Expression, RNAi and CRISPR Genomic Tools and Services","publisher":{"@id":"https:\/\/skyangbio.com\/wordpress\/#organization"},"potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/skyangbio.com\/wordpress\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"},{"@type":"Organization","@id":"https:\/\/skyangbio.com\/wordpress\/#organization","name":"Skyang Bio","url":"https:\/\/skyangbio.com\/wordpress\/","logo":{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/skyangbio.com\/wordpress\/#\/schema\/logo\/image\/","url":"https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/cropped-Skyang-Bio_Logo_New.png","contentUrl":"https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/cropped-Skyang-Bio_Logo_New.png","width":364,"height":283,"caption":"Skyang Bio"},"image":{"@id":"https:\/\/skyangbio.com\/wordpress\/#\/schema\/logo\/image\/"}}]}},"jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/skyangbio.com\/wordpress\/wp-json\/wp\/v2\/pages\/304911","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/skyangbio.com\/wordpress\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/skyangbio.com\/wordpress\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/skyangbio.com\/wordpress\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/skyangbio.com\/wordpress\/wp-json\/wp\/v2\/comments?post=304911"}],"version-history":[{"count":4,"href":"https:\/\/skyangbio.com\/wordpress\/wp-json\/wp\/v2\/pages\/304911\/revisions"}],"predecessor-version":[{"id":304915,"href":"https:\/\/skyangbio.com\/wordpress\/wp-json\/wp\/v2\/pages\/304911\/revisions\/304915"}],"wp:attachment":[{"href":"https:\/\/skyangbio.com\/wordpress\/wp-json\/wp\/v2\/media?parent=304911"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}