{"id":306335,"date":"2026-02-28T11:38:00","date_gmt":"2026-02-28T17:38:00","guid":{"rendered":"https:\/\/skyangbio.com\/wordpress\/?page_id=306335"},"modified":"2026-02-28T14:12:31","modified_gmt":"2026-02-28T20:12:31","slug":"rnai-shrna-target-gene-sets","status":"publish","type":"page","link":"https:\/\/skyangbio.com\/wordpress\/rnai-shrna-target-gene-sets\/","title":{"rendered":"RNAi-shRNA Target Gene Sets"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-page\" data-elementor-id=\"306335\" class=\"elementor elementor-306335\" data-elementor-post-type=\"page\">\n\t\t\t\t<div class=\"elementor-element elementor-element-ef9509e e-flex e-con-boxed e-con e-parent\" data-id=\"ef9509e\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t<div class=\"elementor-element elementor-element-ebbf732 e-con-full e-flex e-con e-child\" data-id=\"ebbf732\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-982d69c elementor-widget elementor-widget-spacer\" data-id=\"982d69c\" data-element_type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-20a5a81 elementor-widget elementor-widget-text-editor\" data-id=\"20a5a81\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p style=\"text-align: center;\">SkySilence\u2122 shRNA<\/p><p style=\"text-align: center;\">Target Gene Sets<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-fc92566 elementor-widget elementor-widget-heading\" data-id=\"fc92566\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Sensor-based shERWOOD Algorithm + UltramiR Scaffold = Best Potency and Specificity<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-71349ee e-con-full e-flex e-con e-child\" data-id=\"71349ee\" data-element_type=\"container\">\n\t\t<div class=\"elementor-element elementor-element-4b32b5a e-con-full e-flex e-con e-child\" data-id=\"4b32b5a\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-a711536 elementor-widget elementor-widget-image\" data-id=\"a711536\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"934\" height=\"784\" src=\"https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/RNAi-shRNA-Target-Gene-Sets_Product-image.png\" class=\"attachment-full size-full wp-image-306994\" alt=\"\" srcset=\"https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/RNAi-shRNA-Target-Gene-Sets_Product-image.png 934w, https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/RNAi-shRNA-Target-Gene-Sets_Product-image-300x252.png 300w, https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/RNAi-shRNA-Target-Gene-Sets_Product-image-768x645.png 768w, https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/RNAi-shRNA-Target-Gene-Sets_Product-image-200x168.png 200w\" sizes=\"(max-width: 934px) 100vw, 934px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-4fa960b e-con-full e-flex e-con e-parent\" data-id=\"4fa960b\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-6bba198 elementor-widget elementor-widget-spacer\" data-id=\"6bba198\" data-element_type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-d40a46c elementor-widget elementor-widget-text-editor\" data-id=\"d40a46c\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>SkySilence Target Gene Sets are available in a flexible portfolio of lentiviral and retroviral vectors with multiple promoter options including doxycycline-inducible systems along with selectable markers and fluorescent reporters. All shRNAs are designed using the <span data-start=\"326\" data-end=\"349\">shERWOOD\u2122 algorithm<\/span>, developed from functional testing of over <span data-start=\"393\" data-end=\"414\">250,000 sequences<\/span>, to deliver potent, single-copy gene knockdown with confidence. The constructs uses an alternate microRNA scaffold called \u201cUltramiR\u201c. The UltramiR scaffold has been optimized for increased shRNA processing and potency based on the key determinants for primary microRNA processing (Auyeung et al 2013).<\/p><ul><li>shERWOOD Algorithm includes filters to minimize off-target effects<\/li><li>Enhanced microRNA scaffold &#8211; increased small RNA processing<\/li><li>All shRNA designs are scored and ranked<\/li><\/ul><p><span style=\"color: #05395e;\"><strong>Deliverable:\u00a0<\/strong><\/span><\/p><ul><li style=\"list-style-type: none;\"><ul><li>3 unique shRNA constructs targeting your gene of interest<\/li><li>Negative control<\/li><li>Glycerol stock, Lentiviral Particles. or Plasmid DNA<\/li><\/ul><\/li><\/ul><p><span style=\"color: #05395e;\"><strong>Timeline:<\/strong><\/span> 2-3 weeks for glycerol stock. Additional 2 weeks for lentiviral particles<\/p><p><span style=\"color: #05395e;\"><strong>Price:<\/strong><\/span> Starting at $775 for glycerol stock<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-0e46590 elementor-widget elementor-widget-spacer\" data-id=\"0e46590\" data-element_type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-ff0eea4 elementor-widget elementor-widget-text-editor\" data-id=\"ff0eea4\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p style=\"text-align: center;\">Request a quote for shRNA target gene set<\/p>\n<p style=\"text-align: center;\">targeting your gene of interest<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-78aa90f elementor-align-center elementor-widget elementor-widget-button\" data-id=\"78aa90f\" data-element_type=\"widget\" data-widget_type=\"button.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-button-wrapper\">\n\t\t\t\t\t<a class=\"elementor-button elementor-button-link elementor-size-sm\" href=\"https:\/\/skyangbio.com\/wordpress\/request-a-quote-rnai-shrna-target-gene-sets\/\">\n\t\t\t\t\t\t<span class=\"elementor-button-content-wrapper\">\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-button-text\">Request a Quote<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-58d1af1 e-flex e-con-boxed e-con e-parent\" data-id=\"58d1af1\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-962730c elementor-widget elementor-widget-heading\" data-id=\"962730c\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Available Vectors<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-8db08df e-flex e-con-boxed e-con e-parent\" data-id=\"8db08df\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t<div class=\"elementor-element elementor-element-2eb6391 e-con-full e-flex e-con e-child\" data-id=\"2eb6391\" data-element_type=\"container\">\n\t\t<div class=\"elementor-element elementor-element-2a945c5 e-con-full e-flex e-con e-child\" data-id=\"2a945c5\" data-element_type=\"container\">\n\t\t<div class=\"elementor-element elementor-element-8cd8c93 e-con-full e-flex e-con e-child\" data-id=\"8cd8c93\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-f3ca7e5 elementor-widget elementor-widget-text-editor\" data-id=\"f3ca7e5\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p style=\"text-align: center;\">Lentiviral Vector Options<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-f6c826c elementor-widget elementor-widget-image\" data-id=\"f6c826c\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" width=\"768\" height=\"197\" src=\"https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/shRNA-Lentiviral-Vector-Option_schematic-768x197.png\" class=\"attachment-medium_large size-medium_large wp-image-306714\" alt=\"\" srcset=\"https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/shRNA-Lentiviral-Vector-Option_schematic-768x197.png 768w, https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/shRNA-Lentiviral-Vector-Option_schematic-300x77.png 300w, https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/shRNA-Lentiviral-Vector-Option_schematic-1024x263.png 1024w, https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/shRNA-Lentiviral-Vector-Option_schematic-1536x395.png 1536w, https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/shRNA-Lentiviral-Vector-Option_schematic-2048x526.png 2048w, https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/shRNA-Lentiviral-Vector-Option_schematic-200x51.png 200w\" sizes=\"(max-width: 768px) 100vw, 768px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-025d900 e-con-full e-flex e-con e-child\" data-id=\"025d900\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-2762e77 elementor-widget elementor-widget-text-editor\" data-id=\"2762e77\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p style=\"text-align: center;\">Inducible Lentiviral Vector Options<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-172e04a elementor-widget elementor-widget-image\" data-id=\"172e04a\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" width=\"768\" height=\"112\" src=\"https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/shRNA-Inducible-Lentiviral-Options_schematic-768x112.png\" class=\"attachment-medium_large size-medium_large wp-image-307324\" alt=\"\" srcset=\"https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/shRNA-Inducible-Lentiviral-Options_schematic-768x112.png 768w, https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/shRNA-Inducible-Lentiviral-Options_schematic-300x44.png 300w, https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/shRNA-Inducible-Lentiviral-Options_schematic-1024x149.png 1024w, https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/shRNA-Inducible-Lentiviral-Options_schematic-1536x224.png 1536w, https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/shRNA-Inducible-Lentiviral-Options_schematic-2048x298.png 2048w, https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/shRNA-Inducible-Lentiviral-Options_schematic-200x29.png 200w\" sizes=\"(max-width: 768px) 100vw, 768px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-26b270e e-con-full e-flex e-con e-child\" data-id=\"26b270e\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-4e9f2b6 elementor-widget elementor-widget-text-editor\" data-id=\"4e9f2b6\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p style=\"text-align: center;\">Retroviral Vector Options<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-35a506f elementor-widget elementor-widget-text-editor\" data-id=\"35a506f\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p style=\"text-align: center;\"><strong>pLMN Vector<\/strong><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-a0c1d65 elementor-widget elementor-widget-image\" data-id=\"a0c1d65\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"768\" height=\"71\" src=\"https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/pLMN-vector-schematic-768x71.png\" class=\"attachment-medium_large size-medium_large wp-image-306598\" alt=\"\" srcset=\"https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/pLMN-vector-schematic-768x71.png 768w, https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/pLMN-vector-schematic-300x28.png 300w, https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/pLMN-vector-schematic-1024x95.png 1024w, https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/pLMN-vector-schematic-1536x143.png 1536w, https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/pLMN-vector-schematic-200x19.png 200w, https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/pLMN-vector-schematic.png 1666w\" sizes=\"(max-width: 768px) 100vw, 768px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-9815fa6 elementor-widget elementor-widget-spacer\" data-id=\"9815fa6\" data-element_type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-7316afc elementor-widget elementor-widget-text-editor\" data-id=\"7316afc\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p style=\"text-align: center;\"><strong>pLMP-d Vector<\/strong><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-d7915f5 elementor-widget elementor-widget-image\" data-id=\"d7915f5\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" src=\"https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/elementor\/thumbs\/pLMP-d-vector-schematic-rge0mxtcbrbrkwmcawuw8ytzicqdngwyog18kz4t8g.png\" title=\"pLMP-d vector schematic\" alt=\"pLMP-d vector schematic\" loading=\"lazy\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-787f974 e-con-full e-flex e-con e-parent\" data-id=\"787f974\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-af87960 elementor-widget elementor-widget-spacer\" data-id=\"af87960\" data-element_type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-a7f9267 elementor-widget elementor-widget-text-editor\" data-id=\"a7f9267\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p style=\"text-align: center;\">SkySilence\u2122 shRNA Whole Genome Arrayed Library<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-645ed11 elementor-widget elementor-widget-heading\" data-id=\"645ed11\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Reliable arrayed shRNA for systematic functional genomics<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-ce42719 elementor-widget elementor-widget-text-editor\" data-id=\"ce42719\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong>SkySilence\u2122 shRNA arrayed libraries<\/strong> are a great resource for core labs. All shRNA constructs are designed using the shERWOOD algorithm which is based on the functional testing of over 250,000 shRNA sequences using a high-throughput sensor assay (Knott et al 2014) and uses key sequence characteristics for predicting shRNA potency to select the rare shRNA designs that are potent at single copy representation in the genome. \u00a0The library uses an alternate microRNA scaffold called \u201cUltramiR\u201c. The UltramiR scaffold has been optimized for increased shRNA processing and potency based on the key determinants for primary microRNA processing (Auyeung et al 2013). Over <strong>18,000<\/strong> human genes targeted. Each human gene is targeted by an average of\u00a0<strong>6<\/strong>\u00a0shRNA constructs<\/p><p><span style=\"color: #05395e;\"><strong>Deliverable:\u00a0<\/strong><\/span><\/p><ul><li style=\"list-style-type: none;\"><ul><li><strong>1,125<\/strong>\u00a096-well glycerol stock plates with barcoded labels<\/li><li>Data file for 96-well plate clone mapping<\/li><li>Also available as pooled format<\/li><\/ul><\/li><\/ul><p><span style=\"color: #05395e;\"><strong>Timeline:<\/strong><\/span> 4-6 weeks<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-2cf6684 elementor-widget elementor-widget-text-editor\" data-id=\"2cf6684\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Library comes in retroviral vector that contains a Neomycin selection marker and a ZsGreen fluorescent reporter<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-89ec9ee elementor-widget elementor-widget-image\" data-id=\"89ec9ee\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"768\" height=\"71\" src=\"https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/pLMN-vector-schematic-768x71.png\" class=\"attachment-medium_large size-medium_large wp-image-306598\" alt=\"\" srcset=\"https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/pLMN-vector-schematic-768x71.png 768w, https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/pLMN-vector-schematic-300x28.png 300w, https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/pLMN-vector-schematic-1024x95.png 1024w, https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/pLMN-vector-schematic-1536x143.png 1536w, https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/pLMN-vector-schematic-200x19.png 200w, https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/pLMN-vector-schematic.png 1666w\" sizes=\"(max-width: 768px) 100vw, 768px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-90f1133 elementor-widget elementor-widget-spacer\" data-id=\"90f1133\" data-element_type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-af5cf49 elementor-widget elementor-widget-text-editor\" data-id=\"af5cf49\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p style=\"text-align: center;\">Request a quote for shRNA<\/p><p style=\"text-align: center;\">Whole Genome Arrayed Library<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-87bbcd9 elementor-align-center elementor-widget elementor-widget-button\" data-id=\"87bbcd9\" data-element_type=\"widget\" data-widget_type=\"button.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-button-wrapper\">\n\t\t\t\t\t<a class=\"elementor-button elementor-button-link elementor-size-sm\" href=\"https:\/\/skyangbio.com\/wordpress\/request-a-quote-shrna-whole-genome-library\/\">\n\t\t\t\t\t\t<span class=\"elementor-button-content-wrapper\">\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-button-text\">Request a Quote<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-b1bae8e elementor-widget elementor-widget-text-editor\" data-id=\"b1bae8e\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p style=\"text-align: left;\">Need a custom screening library cloned into one of our available vectors or a vector you provide to us? We offer custom pooled and arrayed libraries to suit your specific need. Visit <a href=\"https:\/\/skyangbio.com\/wordpress\/custom-screening-libraries\/\">Custom Pooled and Arrayed Screening Libraries<\/a> or Contact us at <a href=\"mailto:products@skyangbio.com\">products@skyangbio.com<\/a> for more information<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-0055aee e-grid e-con-boxed e-con e-parent\" data-id=\"0055aee\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-4ae03e2 elementor-widget elementor-widget-heading\" data-id=\"4ae03e2\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Documents<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-a41e94b e-flex e-con-boxed e-con e-parent\" data-id=\"a41e94b\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t<div class=\"elementor-element elementor-element-6d288ba e-con-full e-flex e-con e-child\" data-id=\"6d288ba\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-debcf17 elementor-widget elementor-widget-image\" data-id=\"debcf17\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/A-Computational-Algorithm-to-Predict-shRNA-Potency-Knott-et-al-2014.pdf\" target=\"_blank\">\n\t\t\t\t\t\t\t<img decoding=\"async\" src=\"https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/elementor\/thumbs\/Molecular-Cell_Pub-image_shERWOOD-shRNA-algorithm-rif0fp674indb7tpzao5kvluyevoc7ztxnzy0vx6v4.png\" title=\"Molecular Cell_Pub image_shERWOOD shRNA algorithm\" alt=\"Molecular Cell_Pub image_shERWOOD shRNA algorithm\" loading=\"lazy\" \/>\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-218ac52 elementor-widget elementor-widget-text-editor\" data-id=\"218ac52\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p class=\"heading-title\" style=\"text-align: center;\"><a href=\"https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/A-Computational-Algorithm-to-Predict-shRNA-Potency-Knott-et-al-2014.pdf\"><span style=\"color: #05395e;\">&#8220;A Computational Algorithm to Predict shRNA Potency&#8221;<\/span><\/a><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-fe9ab60 e-flex e-con-boxed e-con e-parent\" data-id=\"fe9ab60\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-1f796e4 elementor-widget elementor-widget-heading\" data-id=\"1f796e4\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Related Products<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-a49301c elementor-widget elementor-widget-spacer\" data-id=\"a49301c\" data-element_type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-ee9099b elementor-widget elementor-widget-text-editor\" data-id=\"ee9099b\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"color: #05395e;\"><strong><a href=\"https:\/\/skyangbio.com\/wordpress\/custom-screening-libraries\/\">Custom shRNA pooled and arrayed screening libraries<\/a> &#8211; <\/strong><\/span>We can tailor your custom shRNA pooled library or arrayed library to your project specifications. Sequence-verified shRNA constructs are cherry picked from our whole genome arrayed library to target your genes of interest. Stringent quality control is maintained throughout our process.\u00a0\u00a0 All pools go through a stringent Next-Gen sequencing QC to confirm all constructs in the screening pool are represented in an equal manner.\u00a0<\/p><p><span style=\"color: #05395e;\"><strong><a href=\"https:\/\/skyangbio.com\/wordpress\/cloning-vectors\/\">Cloning Vectors<\/a> &#8211; <\/strong><\/span>Our cloning vectors provide robust modular flexibility, allowing the incorporation of various molecular tags and antibiotic-resistant genes.<\/p><p><span style=\"color: #05395e;\"><strong><a href=\"https:\/\/skyangbio.com\/wordpress\/custom-vector-construction\/\">Custom Vector Construction Service<\/a> &#8211; <\/strong><\/span>Skyang will engineer your next custom vector. Choose from our highly customizable inventory of lentiviral and retroviral cloning vectors. If you need a vector that we currently do not offer, then we can generate that vector for you.<\/p><p><span style=\"color: #05395e;\"><strong><a href=\"https:\/\/skyangbio.com\/wordpress\/lentiviral-packaging-services\/\">Lentiviral Packaging Service<\/a> &#8211; <\/strong><\/span>Skyang lentiviral packaging workflow produces high-quality lentiviral particles. All lentiviral particles are functionally titered, which is the gold standard for quality control.\u00a0 This facilitates the most precise calculation of MOI for optimized transduction.<\/p><p><span style=\"color: #05395e;\"><strong><a href=\"https:\/\/skyangbio.com\/wordpress\/ngs-and-deconvolution-service\/\">Custom NGS and Deconvolution Service<\/a> &#8211; <\/strong><\/span>Skyang can help analyze results from your shRNA pooled screening experiments.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>SkySilence\u2122 shRNA Target Gene Sets Sensor-based shERWOOD Algorithm + UltramiR Scaffold = Best Potency and Specificity SkySilence Target Gene Sets&#8230;<\/p>\n","protected":false},"author":15,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-306335","page","type-page","status-publish","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v25.9 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>RNAi-shRNA Target Gene Sets - Skyang Bio<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/skyangbio.com\/wordpress\/rnai-shrna-target-gene-sets\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"RNAi-shRNA Target Gene Sets - Skyang Bio\" \/>\n<meta property=\"og:description\" content=\"SkySilence\u2122 shRNA Target Gene Sets Sensor-based shERWOOD Algorithm + UltramiR Scaffold = Best Potency and Specificity SkySilence Target Gene Sets...\" \/>\n<meta property=\"og:url\" content=\"https:\/\/skyangbio.com\/wordpress\/rnai-shrna-target-gene-sets\/\" \/>\n<meta property=\"og:site_name\" content=\"Skyang Bio\" \/>\n<meta property=\"article:modified_time\" content=\"2026-02-28T20:12:31+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/RNAi-shRNA-Target-Gene-Sets_Product-image.png\" \/>\n\t<meta property=\"og:image:width\" content=\"934\" \/>\n\t<meta property=\"og:image:height\" content=\"784\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/png\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"5 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/skyangbio.com\/wordpress\/rnai-shrna-target-gene-sets\/\",\"url\":\"https:\/\/skyangbio.com\/wordpress\/rnai-shrna-target-gene-sets\/\",\"name\":\"RNAi-shRNA Target Gene Sets - 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