{"id":306772,"date":"2026-02-28T11:03:24","date_gmt":"2026-02-28T17:03:24","guid":{"rendered":"https:\/\/skyangbio.com\/wordpress\/?page_id=306772"},"modified":"2026-02-28T14:11:09","modified_gmt":"2026-02-28T20:11:09","slug":"crispr-target-gene-sets","status":"publish","type":"page","link":"https:\/\/skyangbio.com\/wordpress\/crispr-target-gene-sets\/","title":{"rendered":"CRISPR Target Gene Sets"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-page\" data-elementor-id=\"306772\" class=\"elementor elementor-306772\" data-elementor-post-type=\"page\">\n\t\t\t\t<div class=\"elementor-element elementor-element-e751964 e-flex e-con-boxed e-con e-parent\" data-id=\"e751964\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t<div class=\"elementor-element elementor-element-f8a49b2 e-con-full e-flex e-con e-child\" data-id=\"f8a49b2\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-756e98d elementor-widget elementor-widget-spacer\" data-id=\"756e98d\" data-element_type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-f136fd0 elementor-widget elementor-widget-heading\" data-id=\"f136fd0\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h1 class=\"elementor-heading-title elementor-size-default\"><p style=\"margin-bottom: 25px;font-style: normal;letter-spacing: 0.4px\">SkyEDIT\u2122 CRISPR gRNA<\/p><span style=\"font-style: normal;letter-spacing: 0.4px\">Target Gene Sets<\/span><\/h1>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-9616373 elementor-widget elementor-widget-heading\" data-id=\"9616373\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Optimized gRNA designs, versatile vectors and flexible formats for efficient gene editing<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-12a66df e-con-full e-flex e-con e-child\" data-id=\"12a66df\" data-element_type=\"container\">\n\t\t<div class=\"elementor-element elementor-element-27db754 e-con-full e-flex e-con e-child\" data-id=\"27db754\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-92fa114 elementor-widget elementor-widget-image\" data-id=\"92fa114\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"407\" height=\"345\" src=\"https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/CRISPR-Target-Gene-Sets_product-image.png\" class=\"attachment-full size-full wp-image-306884\" alt=\"\" srcset=\"https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/CRISPR-Target-Gene-Sets_product-image.png 407w, https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/CRISPR-Target-Gene-Sets_product-image-300x254.png 300w, https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/CRISPR-Target-Gene-Sets_product-image-200x170.png 200w\" sizes=\"(max-width: 407px) 100vw, 407px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-e811140 e-con-full e-flex e-con e-parent\" data-id=\"e811140\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-ab68235 elementor-widget elementor-widget-spacer\" data-id=\"ab68235\" data-element_type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-354404e elementor-widget elementor-widget-text-editor\" data-id=\"354404e\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>SkyEDIT CRISPR-Cas9 gRNA Target gene sets are available in a range of vectors including CRISPRko (knockout) single gRNA or gRNA+Cas9, CRISPRa (activation), and CRISPRi (interference). The sgRNA vectors are designed to select for high gene-editing efficiency using direct indicators of CRISPR-Cas9 expression from fluorescent reporters or selection markers. The Croatan algorithm was used to design all gRNAs or we can use your specific gRNA designs upon request.<\/p><ul><li>CRoatan algorithm gRNA designs result in superior knockout efficiency<\/li><li>sgRNA lentiviral vectors include versatile promoter, selection marker and fluorescent reporter options\u00a0<\/li><li>All clones are sequence-verified<\/li><\/ul><p><span style=\"color: #05395e;\"><strong>Deliverable:\u00a0<\/strong><\/span><\/p><ul><li style=\"list-style-type: none;\"><ul><li>3 unique gRNA constructs targeting your gene of interest<\/li><li>Negative control<\/li><li>Glycerol stock, Lentiviral Particles. or Plasmid DNA<\/li><\/ul><\/li><\/ul><p><span style=\"color: #05395e;\"><strong>Timeline:<\/strong><\/span> 2-3 weeks for glycerol stock. Additional 2 weeks for lentiviral particles<\/p><p><span style=\"color: #05395e;\"><strong>Price:<\/strong><\/span> Starting at $775 for glycerol stock<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-f8d03cc elementor-widget elementor-widget-spacer\" data-id=\"f8d03cc\" data-element_type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-ff022a6 elementor-widget elementor-widget-text-editor\" data-id=\"ff022a6\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p style=\"text-align: center;\">Request a quote for CRISPR gRNA target gene set<\/p><p style=\"text-align: center;\">targeting your gene of interest<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-b2df2f6 elementor-align-center elementor-widget elementor-widget-button\" data-id=\"b2df2f6\" data-element_type=\"widget\" data-widget_type=\"button.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-button-wrapper\">\n\t\t\t\t\t<a class=\"elementor-button elementor-button-link elementor-size-sm\" href=\"https:\/\/skyangbio.com\/wordpress\/request-a-quote-crispr-grna-target-gene-sets\/\">\n\t\t\t\t\t\t<span class=\"elementor-button-content-wrapper\">\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-button-text\">Request a Quote<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-43ac7f4 e-flex e-con-boxed e-con e-parent\" data-id=\"43ac7f4\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-47990d3 elementor-widget elementor-widget-heading\" data-id=\"47990d3\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Available Vectors<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-65d52c7 e-flex e-con-boxed e-con e-parent\" data-id=\"65d52c7\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t<div class=\"elementor-element elementor-element-8539f9f e-con-full e-flex e-con e-child\" data-id=\"8539f9f\" data-element_type=\"container\">\n\t\t<div class=\"elementor-element elementor-element-24610b8 e-con-full e-flex e-con e-child\" data-id=\"24610b8\" data-element_type=\"container\">\n\t\t<div class=\"elementor-element elementor-element-4cb03dd e-con-full e-flex e-con e-child\" data-id=\"4cb03dd\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-27213e9 elementor-widget elementor-widget-text-editor\" data-id=\"27213e9\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p style=\"text-align: center;\">Single gRNA Vector Options<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-b06653b elementor-widget elementor-widget-image\" data-id=\"b06653b\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" src=\"https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/elementor\/thumbs\/CRISPR-gRNA-single_vector-schematic-rhzhb6o1ja96l35b3j22c7luo4siehbck7eo9e20ao.png\" title=\"CRISPR-gRNA-single_vector schematic\" alt=\"CRISPR-gRNA-single_vector schematic\" loading=\"lazy\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-08d6558 e-con-full e-flex e-con e-child\" data-id=\"08d6558\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-bfd6b90 elementor-widget elementor-widget-text-editor\" data-id=\"bfd6b90\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p style=\"text-align: center;\">All-in-one gRNA + Cas9 Vector Options<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-48e13df elementor-widget elementor-widget-image\" data-id=\"48e13df\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" src=\"https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/elementor\/thumbs\/CRISPR-gRNA-ALL_vector-schematic-rhzhbnl4yavefboeyg5v83gbxa8xxnp5ijmlxnq3nk.png\" title=\"CRISPR-gRNA-ALL_vector schematic\" alt=\"CRISPR-gRNA-ALL_vector schematic\" loading=\"lazy\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-a5cf8d2 e-flex e-con-boxed e-con e-parent\" data-id=\"a5cf8d2\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t<div class=\"elementor-element elementor-element-c041ca3 e-con-full e-flex e-con e-child\" data-id=\"c041ca3\" data-element_type=\"container\">\n\t\t<div class=\"elementor-element elementor-element-49adb45 e-con-full e-flex e-con e-child\" data-id=\"49adb45\" data-element_type=\"container\">\n\t\t<div class=\"elementor-element elementor-element-d58142b e-con-full e-flex e-con e-child\" data-id=\"d58142b\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-610419f elementor-widget elementor-widget-text-editor\" data-id=\"610419f\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p style=\"text-align: center;\">CRISPRa gRNA Vector Options<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-2ef1dd3 elementor-widget elementor-widget-image\" data-id=\"2ef1dd3\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" src=\"https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/elementor\/thumbs\/CRISPRa-gRNA-ALL_dCas9-VPR-vector-schematic-rhzhdl5701jk97np2kfqpls9bkya2munk3fceuhse8.png\" title=\"CRISPRa-gRNA-ALL_dCas9-VPR vector schematic\" alt=\"CRISPRa-gRNA-ALL_dCas9-VPR vector schematic\" loading=\"lazy\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-774a6a7 e-con-full e-flex e-con e-child\" data-id=\"774a6a7\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-3c4222c elementor-widget elementor-widget-text-editor\" data-id=\"3c4222c\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p style=\"text-align: center;\">CRISPRi gRNA Vector Options<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-ca54150 elementor-widget elementor-widget-image\" data-id=\"ca54150\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" src=\"https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/elementor\/thumbs\/CRISPRi-gRNA-ALL_dCas9-KRAB-vector-schematic-rhzhebgobeipkkptkyuzfelncwr1l16syt6apial8g.png\" title=\"CRISPRi-gRNA-ALL_dCas9-KRAB vector schematic\" alt=\"CRISPRi-gRNA-ALL_dCas9-KRAB vector schematic\" loading=\"lazy\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-77f6708 e-con-full e-flex e-con e-parent\" data-id=\"77f6708\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-83b7442 elementor-widget elementor-widget-spacer\" data-id=\"83b7442\" data-element_type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-9e04eeb elementor-widget elementor-widget-text-editor\" data-id=\"9e04eeb\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p style=\"text-align: center;\">CRISPR Dual gRNA Vector Options<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-e739e5b elementor-widget elementor-widget-spacer\" data-id=\"e739e5b\" data-element_type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-fb5f91f elementor-widget elementor-widget-image\" data-id=\"fb5f91f\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" src=\"https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/elementor\/thumbs\/CRISPR-gRNA-dual_vector-schematic-rjruxuvswqarsr2gttr62bpxi42vqm0t9o2r0a9sz4.png\" title=\"CRISPR-gRNA-dual_vector schematic\" alt=\"CRISPR-gRNA-dual_vector schematic\" loading=\"lazy\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-635de91 e-con-full e-flex e-con e-parent\" data-id=\"635de91\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-79c12a3 elementor-widget elementor-widget-spacer\" data-id=\"79c12a3\" data-element_type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-7248973 elementor-widget elementor-widget-text-editor\" data-id=\"7248973\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p style=\"text-align: center;\">CRISPR ccdB Cloning Vector<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-6d3d072 elementor-widget elementor-widget-spacer\" data-id=\"6d3d072\" data-element_type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-9744cf3 elementor-widget elementor-widget-image\" data-id=\"9744cf3\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" src=\"https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/elementor\/thumbs\/CRISPR-ccdB-vector-schematic-rhzhgzcezmsbp7m0puc4m9i9b3fycttfaaazgll9gw.png\" title=\"CRISPR-ccdB vector schematic\" alt=\"CRISPR-ccdB vector schematic\" loading=\"lazy\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-06a7be3 elementor-widget elementor-widget-text-editor\" data-id=\"06a7be3\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>The\u00a0<strong>ccdB cassette<\/strong>\u00a0is inserted between the\u00a0<strong>U6 promoter<\/strong>\u00a0and the\u00a0<strong>gRNA<\/strong>, which is removed upon insertion of the target gRNA. Because the ccdB vector cannot survive in standard competent cells used for sgRNA cloning, this design significantly minimizes background contamination and improves the efficiency of obtaining correct gRNA clones.<\/p><p data-start=\"0\" data-end=\"160\">This CRISPR ccdB cloning vector is ideal for researchers who need reliable, high-throughput-compatible CRISPR cloning to support both targeted experiments and large-scale genetic studies.<\/p><ul data-start=\"162\" data-end=\"1097\"><li data-start=\"162\" data-end=\"276\"><p data-start=\"164\" data-end=\"276\">Gene knockout\/knock-in studies, pathway analysis, and functional validation<\/p><\/li><li data-start=\"277\" data-end=\"374\"><p data-start=\"279\" data-end=\"374\">Interrogation of gene function in cellular processes and phenotypic assays<\/p><\/li><li data-start=\"375\" data-end=\"475\"><p data-start=\"377\" data-end=\"475\">Targeted gene perturbation studies and CRISPR-based screens<\/p><\/li><li data-start=\"476\" data-end=\"580\"><p data-start=\"478\" data-end=\"580\">Identification and validation of oncogenes, tumor suppressors, and drug targets<\/p><\/li><li data-start=\"581\" data-end=\"683\"><p data-start=\"583\" data-end=\"683\">Gene editing in immune cells to study signaling, activation, and immune responses<\/p><\/li><li data-start=\"791\" data-end=\"897\"><p data-start=\"793\" data-end=\"897\">Studying gene regulation during differentiation<\/p><\/li><li data-start=\"898\" data-end=\"994\"><p data-start=\"900\" data-end=\"994\">Genome engineering in pluripotent or differentiated stem cell models<\/p><\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-2cc9b6c e-con-full e-flex e-con e-parent\" data-id=\"2cc9b6c\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-9b4b5d3 elementor-widget elementor-widget-spacer\" data-id=\"9b4b5d3\" data-element_type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-56f5edc elementor-widget elementor-widget-text-editor\" data-id=\"56f5edc\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p style=\"text-align: center;\">SkyEDIT\u2122 Dual gRNA Whole Genome Arrayed Library<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-88a53e8 elementor-widget elementor-widget-heading\" data-id=\"88a53e8\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Dual gRNA lentiviral vector developed using the CRoatan gRNA design algorithm for superior knockout efficiency<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-f2db3e5 elementor-widget elementor-widget-text-editor\" data-id=\"f2db3e5\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong>Skyang Bio\u2019s SkyEDIT\u2122 dual gRNA CRISPR arrayed library<\/strong> was developed in collaboration with Cold Spring Harbor Laboratory. The dual gRNA design increases the likelihood of CRISPR Cas9 cuts and frameshifts for efficient gene knockout. Each lentiviral construct expresses 2 gRNAs targeting the same gene, where all gRNAs have been designed using the CRoatan algorithm (<a href=\"https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/A-CRISPR-Resource-for-Individual-Combinatorial-or-Multiplex-Gene-Knockout-Erard-et-al.-2017.pdf\"><u><b>read publication<\/b><\/u><\/a>). Each dual gRNA construct contains a unique barcode, a puromycin selection marker and a GFP fluorescent reporter<\/p><ul><li>Over 18,000 human genes targeted<\/li><li>Each human gene is targeted by 3-5 unique dual gRNA constructs<\/li><li>Every dual gRNA construct has been sequence-verified<\/li><li>Over 1,000 negative controls are contained in the arrayed library<\/li><\/ul><p><span style=\"color: #05395e;\"><strong>Deliverable:\u00a0<\/strong><\/span><\/p><ul><li style=\"list-style-type: none;\"><ul><li><strong>670<\/strong> 96-well glycerol stock plates with barcoded labels<\/li><li>Datafile for 96-well plate clone mapping<\/li><li>Pooled format available as lentiviral particles or plasmid DNA<\/li><\/ul><\/li><\/ul><p><span style=\"color: #05395e;\"><strong>Timeline:<\/strong><\/span> 4-6 weeks<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-a6e474a elementor-widget elementor-widget-text-editor\" data-id=\"a6e474a\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Library comes in lentiviral vector that contains the SFFV promoter with a puromycin selection marker and a GFP fluorescent reporter<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-267e322 elementor-widget elementor-widget-image\" data-id=\"267e322\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" width=\"768\" height=\"55\" src=\"https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/CRISPR-gRNA-dual_vector-schematic-Array-Library-768x55.png\" class=\"attachment-medium_large size-medium_large wp-image-306817\" alt=\"\" srcset=\"https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/CRISPR-gRNA-dual_vector-schematic-Array-Library-768x55.png 768w, https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/CRISPR-gRNA-dual_vector-schematic-Array-Library-300x21.png 300w, https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/CRISPR-gRNA-dual_vector-schematic-Array-Library-1024x73.png 1024w, https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/CRISPR-gRNA-dual_vector-schematic-Array-Library-1536x110.png 1536w, https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/CRISPR-gRNA-dual_vector-schematic-Array-Library-2048x146.png 2048w, https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/CRISPR-gRNA-dual_vector-schematic-Array-Library-200x14.png 200w\" sizes=\"(max-width: 768px) 100vw, 768px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-3db6d3f elementor-widget elementor-widget-spacer\" data-id=\"3db6d3f\" data-element_type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-fe67b5e elementor-widget elementor-widget-text-editor\" data-id=\"fe67b5e\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p style=\"text-align: center;\">Request a quote for CRISPR Dual gRNA<\/p><p style=\"text-align: center;\">Whole Genome Arrayed Library<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-b06beee elementor-align-center elementor-widget elementor-widget-button\" data-id=\"b06beee\" data-element_type=\"widget\" data-widget_type=\"button.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-button-wrapper\">\n\t\t\t\t\t<a class=\"elementor-button elementor-button-link elementor-size-sm\" href=\"https:\/\/skyangbio.com\/wordpress\/request-a-quote-crispr-dual-grna-whole-genome-arrayed-library\/\">\n\t\t\t\t\t\t<span class=\"elementor-button-content-wrapper\">\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-button-text\">Request a Quote<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-5337463 elementor-widget elementor-widget-text-editor\" data-id=\"5337463\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p style=\"text-align: left;\">Need a custom screening library cloned into one of our available vectors or a vector you provide to us? We offer custom pooled and arrayed libraries to suit your specific need. Visit <a href=\"https:\/\/skyangbio.com\/wordpress\/custom-screening-libraries\/\">Custom Pooled and Arrayed Screening Libraries<\/a> or Contact us at <a href=\"mailto:products@skyangbio.com\">products@skyangbio.com<\/a> for more information<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-118969d e-grid e-con-boxed e-con e-parent\" data-id=\"118969d\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-e96fff2 elementor-widget elementor-widget-heading\" data-id=\"e96fff2\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Documents<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-bf59c2a e-flex e-con-boxed e-con e-parent\" data-id=\"bf59c2a\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t<div class=\"elementor-element elementor-element-cfb85ce e-con-full e-flex e-con e-child\" data-id=\"cfb85ce\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-1331e6c elementor-widget elementor-widget-image\" data-id=\"1331e6c\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"https:\/\/genome.cshlp.org\/content\/19\/12\/2324\" target=\"_blank\">\n\t\t\t\t\t\t\t<img decoding=\"async\" src=\"https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/elementor\/thumbs\/Molecular-Cell_Pub-image_CRoatan-algorithm-ridiqjdlduapm5ula6dc17cx356mq2hahep3348lq8.png\" title=\"Molecular Cell_Pub image_CRoatan algorithm\" alt=\"Molecular Cell_Pub image_CRoatan algorithm\" loading=\"lazy\" \/>\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-305cedd elementor-widget elementor-widget-text-editor\" data-id=\"305cedd\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p class=\"heading-title\" style=\"text-align: center;\"><a href=\"https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/A-CRISPR-Resource-for-Individual-Combinatorial-or-Multiplex-Gene-Knockout-Erard-et-al.-2017.pdf\"><span style=\"color: #05395e;\">&#8220;A CRISPR Resource for Individual, Combinatorial, or Multiplex Gene Knockout&#8221;<\/span><\/a><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-32e1476 e-flex e-con-boxed e-con e-parent\" data-id=\"32e1476\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-893d8bc elementor-widget elementor-widget-heading\" data-id=\"893d8bc\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Related Products<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-9e6bb04 elementor-widget elementor-widget-spacer\" data-id=\"9e6bb04\" data-element_type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-590de89 elementor-widget elementor-widget-text-editor\" data-id=\"590de89\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"color: #05395e;\"><strong><a href=\"https:\/\/skyangbio.com\/wordpress\/custom-screening-libraries\/\">Custom CRISPR gRNA pooled and arrayed screening libraries<\/a> &#8211; <\/strong><\/span>We can build custom CRISPR pooled libraries for your screening projects and genome editing experiments. Stringent quality control is maintained throughout our process.\u00a0 All pools go through a Next-Gen sequencing QC to confirm all constructs in the screening pool are represented in an equal manner. The CRoatan algorithm is used to design gRNAs. Other gRNA design algorithms available upon request.<\/p><p><span style=\"color: #05395e;\"><strong><a href=\"https:\/\/skyangbio.com\/wordpress\/cloning-vectors\/\">Cloning Vectors<\/a> &#8211; <\/strong><\/span>Our cloning vectors provide robust modular flexibility, allowing the incorporation of various molecular tags and antibiotic-resistant genes.<\/p><p><span style=\"color: #05395e;\"><strong><a href=\"https:\/\/skyangbio.com\/wordpress\/custom-vector-construction\/\">Custom Vector Construction<\/a> &#8211; <\/strong><\/span>Skyang will engineer your next custom vector. Choose from our highly customizable inventory of lentiviral and retroviral cloning vectors. If you need a vector that we currently do not offer, then we can generate that vector for you.<\/p><p><span style=\"color: #05395e;\"><strong><a href=\"https:\/\/skyangbio.com\/wordpress\/lentiviral-packaging-services\/\">Lentiviral Packaging Service<\/a> &#8211; <\/strong><\/span>Skyang lentiviral packaging workflow produces high-quality lentiviral particles. All lentiviral particles are functionally titered, which is the gold standard for quality control.\u00a0 This facilitates the most precise calculation of MOI for optimized transduction.<\/p><p><span style=\"color: #05395e;\"><strong><a href=\"https:\/\/skyangbio.com\/wordpress\/ngs-and-deconvolution-service\/\">Custom NGS and Deconvolution Service<\/a> &#8211; <\/strong><\/span>Skyang can help analyze results from your CRISPR pooled screening experiments.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>SkyEDIT\u2122 CRISPR gRNA Target Gene Sets Optimized gRNA designs, versatile vectors and flexible formats for efficient gene editing SkyEDIT CRISPR-Cas9&#8230;<\/p>\n","protected":false},"author":15,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-306772","page","type-page","status-publish","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v25.9 - 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