{"id":306791,"date":"2026-02-28T08:57:10","date_gmt":"2026-02-28T14:57:10","guid":{"rendered":"https:\/\/skyangbio.com\/wordpress\/?page_id=306791"},"modified":"2026-02-28T13:18:43","modified_gmt":"2026-02-28T19:18:43","slug":"ngs-and-deconvolution-service","status":"publish","type":"page","link":"https:\/\/skyangbio.com\/wordpress\/ngs-and-deconvolution-service\/","title":{"rendered":"NGS and Deconvolution Service"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-page\" data-elementor-id=\"306791\" class=\"elementor elementor-306791\" data-elementor-post-type=\"page\">\n\t\t\t\t<div class=\"elementor-element elementor-element-9be38f5 e-flex e-con-boxed e-con e-parent\" data-id=\"9be38f5\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t<div class=\"elementor-element elementor-element-9778db3 e-con-full e-flex e-con e-child\" data-id=\"9778db3\" data-element_type=\"container\">\n\t\t<div class=\"elementor-element elementor-element-60a4a8d e-con-full e-flex e-con e-child\" data-id=\"60a4a8d\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-7a4fcd1 elementor-widget elementor-widget-spacer\" data-id=\"7a4fcd1\" data-element_type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-2e98d5b elementor-widget elementor-widget-heading\" data-id=\"2e98d5b\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h1 class=\"elementor-heading-title elementor-size-default\"><p style=\"margin-bottom: 25px;font-style: normal;letter-spacing: 0.4px\">NGS and Deconvolution<\/p><span style=\"font-style: normal;letter-spacing: 0.4px\">Service<\/span><\/h1>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-8674d50 elementor-widget elementor-widget-heading\" data-id=\"8674d50\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">From pooled libraries to clear research results<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-01d3edf e-con-full e-flex e-con e-child\" data-id=\"01d3edf\" data-element_type=\"container\">\n\t\t<div class=\"elementor-element elementor-element-33ded16 e-con-full e-flex e-con e-child\" data-id=\"33ded16\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-e3e8b1a elementor-widget elementor-widget-image\" data-id=\"e3e8b1a\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"934\" height=\"784\" src=\"https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/NGS-Deconvolution-Service_Product-image.png\" class=\"attachment-full size-full wp-image-306991\" alt=\"Next Generation Sequencing and Deconvolution Service\" srcset=\"https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/NGS-Deconvolution-Service_Product-image.png 934w, https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/NGS-Deconvolution-Service_Product-image-300x252.png 300w, https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/NGS-Deconvolution-Service_Product-image-768x645.png 768w, https:\/\/skyangbio.com\/wordpress\/wp-content\/uploads\/NGS-Deconvolution-Service_Product-image-200x168.png 200w\" sizes=\"(max-width: 934px) 100vw, 934px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-313c229 e-con-full e-flex e-con e-parent\" data-id=\"313c229\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-ff7764c elementor-widget elementor-widget-text-editor\" data-id=\"ff7764c\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Skyang Bio can help analyze results from your CRISPR or shRNA pooled screening experiments. Send us cell pellets from your screen and we will extract genomic DNA, prepare the samples for Next-Generation Sequencing (NGS), perform NGS on the appropriate NGS platform, and align the gRNA\/shRNA sequence reads against the NGS reads from the screening pool. You can then determine which gRNAs\/shRNAs in your screening pool were enriched or depleted.<\/p>\n<ul>\n<li>Skyang offers the highest quality CRISPR\/shRNA screening libraries for your experiment<\/li>\n<li>Designed to support discovery, functional genomics, and screening studies<\/li>\n<li>High-accuracy sequencing with flexible read lengths and depths<\/li>\n<li>Flexible library preparation for CRISPR and RNAi pooled libraries<\/li>\n<\/ul>\n<div><span style=\"color: #05395e; font-weight: bold;\">Workflow:<\/span><\/div>\n<ul>\n<li>Perform your screens on your cell lines<\/li>\n<li>Send frozen cell pellets to Skyang<\/li>\n<li>We will extract gDNA and prepare the samples for NGS<\/li>\n<li>Skyang will run the samples on the appropriate NGS platform<\/li>\n<\/ul>\n<p><span style=\"color: #05395e;\"><strong>Deliverable:<\/strong><\/span><\/p>\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li>Data comparing the gRNA\/shRNA NGS counts of the screening pool compared to the gRNA\/shRNA counts from the screened samples<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p><span style=\"color: #05395e;\"><strong>Timeline:<\/strong><\/span> 1 week per sample<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-990adf9 elementor-widget elementor-widget-text-editor\" data-id=\"990adf9\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p style=\"text-align: center;\"><span data-olk-copy-source=\"MessageBody\">Fill out our quick request form and<\/span><\/p><p style=\"text-align: center;\"><span data-olk-copy-source=\"MessageBody\">our team will prepare a detailed quote for you<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-aa54820 elementor-widget elementor-widget-spacer\" data-id=\"aa54820\" data-element_type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-bb211bb elementor-align-center elementor-widget elementor-widget-button\" data-id=\"bb211bb\" data-element_type=\"widget\" data-widget_type=\"button.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-button-wrapper\">\n\t\t\t\t\t<a class=\"elementor-button elementor-button-link elementor-size-sm\" href=\"https:\/\/skyangbio.com\/wordpress\/request-a-quote-ngs-and-deconvolution-service\/\">\n\t\t\t\t\t\t<span class=\"elementor-button-content-wrapper\">\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-button-text\">Request a Quote<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-9872476 e-flex e-con-boxed e-con e-parent\" data-id=\"9872476\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-0454fd2 elementor-widget elementor-widget-heading\" data-id=\"0454fd2\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Related Products<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-8c5d782 elementor-widget elementor-widget-text-editor\" data-id=\"8c5d782\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"color: #05395e;\"><strong><a href=\"https:\/\/skyangbio.com\/wordpress\/custom-screening-libraries\/\">Custom CRISPR and shRNA pooled and arrayed screening libraries<\/a> &#8211; <\/strong><\/span>We can tailor your custom CRISPR and shRNA library for gene knockout\/knockdown screening projects and genome editing experiments.<\/p><p><span style=\"color: #05395e;\"><strong><a href=\"https:\/\/skyangbio.com\/wordpress\/custom-vector-construction\/\">Custom Vector Construction<\/a> &#8211; <\/strong><\/span>Skyang will engineer your next custom vector. Choose from our highly customizable inventory of lentiviral and retroviral cloning vectors. If you need a vector that we currently do not offer, then we can generate that vector for you.<\/p><p><span style=\"color: #05395e;\"><strong><a href=\"https:\/\/skyangbio.com\/wordpress\/lentiviral-packaging-services\/\">Lentiviral Packaging Service<\/a> &#8211; <\/strong><\/span>Skyang lentiviral packaging workflow produces high-quality lentiviral particles. All lentiviral particles are functionally titered, which is the gold standard for quality control.\u00a0 This facilitates the most precise calculation of MOI for optimized transduction.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>NGS and Deconvolution Service From pooled libraries to clear research results Skyang Bio can help analyze results from your CRISPR&#8230;<\/p>\n","protected":false},"author":15,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-306791","page","type-page","status-publish","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v25.9 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>NGS and Deconvolution Service - Skyang Bio<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/skyangbio.com\/wordpress\/ngs-and-deconvolution-service\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"NGS and Deconvolution Service - 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