Skyang Bio Service: POOL AND ARRAYED LIBRARY
Skyang Bio provides custom pooled and arrayed shRNA libraries for gene knockdown screening. Contact Skyang Bio today to discuss your target genes, preferred vector, and delivery format.
Highlights
Customer Screening pools
Customers can use any of our shRNA lentiviral vectors for creating custom screening pools.
- Client identifies genes of interest to be targeted (i.e. druggable genes) and desired vector
- Skyang uses its shRNA algorithm to design targeting shRNAs.
- Skyang synthesizes shRNAs and clones them into the vector backbone.
- The screening pool is qualified by Next-Gen Sequencing to ensure equal representation of every construct.
- Skyang packages the screening pool into viral particles and performs functional titer analysis.
Customer Screening Pools QC
Skyang Bio offers a Certificate of Analysis for all screening pools, showing distribution of gRNAs/shRNAs as determined by Nex–Gen Sequencing.
Lentiviral pools will be evaluated by functional viral titer*.
*Skyang Bio performs a functional titer on all lentiviral particles delivered. Functional titer analysis (opposed to physical titer analysis) is a more accurate measurement for calculating MOIs that are used in screening experiments.
Customer Arrayed Library
Streamlined work-flows allow Skyang to be one of the few vendors that offers customized arrayed shRNA libraries in 96-well format