Custom CRISPR and shRNA
Screening Libraries
Arrayed or Pooled libraries provide flexible solutions for your screen
SkyEDIT™ CRISPR gRNA Arrayed and Pooled Libraries
Skyang Bio’s extensive catalog of lentiviral vectors offers complete flexibility when planning your custom CRISPR screen. Our team is dedicated to delivering high-quality, optimized libraries that empower cutting-edge discoveries in functional genomics and therapeutic development. The CRoatan algorithm is used to design gRNAs or we can use your gRNA designs.
- CRISPRko, CRISPRa, or CRISPRi screening libraries are available
- Target your custom gene list or choose whole genome
- Provide the number of gRNAs needed for targeting each gene
- QC is based on NGS to ensure gRNA representation and equal distribution
Deliverable:
Provided in arrayed or pooled format
Plasmid DNA (Maxiprep or Gigaprep) or ready-to-use lentiviral particles
Certificate of analysis showing gRNA distribution according to NGS results
Timeline: As few as 6 weeks for plasmid DNA. Add 2 weeks for lentiviral particles
Request a quote for CRISPR gRNA
arrayed or pooled library
Available CRISPR Vectors
Single gRNA Vector Options
All-in-one gRNA + Cas9 Vector Options
CRISPRa gRNA Vector Options
CRISPRi gRNA Vector Options
CRISPR Dual gRNA Vector Options
SkySilence™ shRNA Arrayed and Pooled Libraries
Skyang Bio provides multiple lentiviral, inducible lentiviral and retroviral vectors for complete flexibility when planning your custom shRNA screen. Our team is dedicated to delivering high-quality, optimized libraries that empower cutting-edge discoveries in functional genomics and therapeutic development. The shERWOOD algorithm is used to design shRNAs or we can use your shRNA designs.
- Lentiviral, Inducible Lentiviral or Retroviral screening libraries are available
- Target your custom gene list or choose whole genome
- Provide the number of shRNAs needed for targeting each gene
- QC is based on NGS to ensure shRNA representation and equal distribution
Deliverable:
Plasmid DNA (Maxiprep or Gigaprep) or ready-to-use lentiviral particles
Certificate of analysis showing shRNA distribution according to NGS results
Timeline: As few as 6 weeks for plasmid DNA. Add 2 weeks for lentiviral particles
Request a quote for shRNA
arrayed or pooled library
Available shRNA Vectors
Lentiviral Vector Options
Inducible Lentiviral Vector Options
Retroviral Vector Options
pLMN Vector
pLMP-d Vector
Related Products
Custom NGS and Deconvolution Service – Skyang can help analyze results from your CRISPR and shRNA pooled screening experiments.
Custom Vector Construction Service – Skyang will engineer your next custom vector. Choose from our highly customizable inventory of lentiviral and retroviral cloning vectors. If you need a vector that we currently do not offer, then we can generate that vector for you.
Lentiviral Packaging Service – Skyang lentiviral packaging workflow produces high-quality lentiviral particles. All lentiviral particles are functionally titered, which is the gold standard for quality control. This facilitates the most precise calculation of MOI for optimized transduction.
Cloning Vectors – Our cloning vectors provide robust modular flexibility, allowing the incorporation of various molecular tags and antibiotic-resistant genes.