SkySilence™ shRNA
Target Gene Sets
Sensor-based shERWOOD Algorithm + UltramiR Scaffold = Best Potency and Specificity
SkySilence Target Gene Sets are available in a flexible portfolio of lentiviral and retroviral vectors with multiple promoter options including doxycycline-inducible systems along with selectable markers and fluorescent reporters. All shRNAs are designed using the shERWOOD™ algorithm, developed from functional testing of over 250,000 sequences, to deliver potent, single-copy gene knockdown with confidence. The constructs uses an alternate microRNA scaffold called “UltramiR“. The UltramiR scaffold has been optimized for increased shRNA processing and potency based on the key determinants for primary microRNA processing (Auyeung et al 2013).
- shERWOOD Algorithm includes filters to minimize off-target effects
- Enhanced microRNA scaffold – increased small RNA processing
- All shRNA designs are scored and ranked
Deliverable:
- 3 unique shRNA constructs targeting your gene of interest
- Negative control
- Glycerol stock, Lentiviral Particles. or Plasmid DNA
Timeline: 2-3 weeks for glycerol stock. Additional 2 weeks for lentiviral particles
Price: Starting at $775 for glycerol stock
Request a quote for shRNA target gene set
targeting your gene of interest
Available Vectors
Lentiviral Vector Options
Inducible Lentiviral Vector Options
Retroviral Vector Options
pLMN Vector
pLMP-d Vector
SkySilence™ shRNA Whole Genome Arrayed Library
Reliable arrayed shRNA for systematic functional genomics
SkySilence™ shRNA arrayed libraries are a great resource for core labs. All shRNA constructs are designed using the shERWOOD algorithm which is based on the functional testing of over 250,000 shRNA sequences using a high-throughput sensor assay (Knott et al 2014) and uses key sequence characteristics for predicting shRNA potency to select the rare shRNA designs that are potent at single copy representation in the genome. The library uses an alternate microRNA scaffold called “UltramiR“. The UltramiR scaffold has been optimized for increased shRNA processing and potency based on the key determinants for primary microRNA processing (Auyeung et al 2013). Over 18,000 human genes targeted. Each human gene is targeted by an average of 6 shRNA constructs
Deliverable:
- 1,125 96-well glycerol stock plates with barcoded labels
- Data file for 96-well plate clone mapping
- Also available as pooled format
Timeline: 4-6 weeks
Library comes in retroviral vector that contains a Neomycin selection marker and a ZsGreen fluorescent reporter
Request a quote for shRNA
Whole Genome Arrayed Library
Need a custom screening library cloned into one of our available vectors or a vector you provide to us? We offer custom pooled and arrayed libraries to suit your specific need. Visit Custom Pooled and Arrayed Screening Libraries or Contact us at products@skyangbio.com for more information
Documents
Related Products
Custom shRNA pooled and arrayed screening libraries – We can tailor your custom shRNA pooled library or arrayed library to your project specifications. Sequence-verified shRNA constructs are cherry picked from our whole genome arrayed library to target your genes of interest. Stringent quality control is maintained throughout our process. All pools go through a stringent Next-Gen sequencing QC to confirm all constructs in the screening pool are represented in an equal manner.
Cloning Vectors – Our cloning vectors provide robust modular flexibility, allowing the incorporation of various molecular tags and antibiotic-resistant genes.
Custom Vector Construction Service – Skyang will engineer your next custom vector. Choose from our highly customizable inventory of lentiviral and retroviral cloning vectors. If you need a vector that we currently do not offer, then we can generate that vector for you.
Lentiviral Packaging Service – Skyang lentiviral packaging workflow produces high-quality lentiviral particles. All lentiviral particles are functionally titered, which is the gold standard for quality control. This facilitates the most precise calculation of MOI for optimized transduction.
Custom NGS and Deconvolution Service – Skyang can help analyze results from your shRNA pooled screening experiments.