cDNA and ORF Clones: Versatile Tools for Gene Overexpression

cDNA and ORF clones are efficient and convenient resources for accessing gene content, enabling a wide range of overexpression applications in cell culture and in vitro studies. These sequences are inserted into expression vectors driven by strong promoters and delivered to target cells through plasmid transfection or lentiviral transduction.

cDNA Clones

  • Retain native regulatory elements, allowing the study of genes in their natural regulatory context.
  • Ideal for applications where preserving endogenous gene regulation is critical, such as transcriptional control studies.

ORF Clones

  • Exclude 5′ and 3′ untranslated regions (UTRs), offering a direct and simplified route to protein expression.
  • Provide a streamlined approach for applications requiring efficient protein production.

Applications

Both cDNA and ORF clones are widely utilized in various research areas, including:

  • Gene overexpression studies.
  • RNAi rescue experiments.
  • Structural and functional protein analysis.
  • Protein localization studies using visualization techniques.
  • Biochemical purification workflows.
  • Identification of protein-protein interactions through co-immunoprecipitation.

Easy to Order

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cDNA & ORF Collections

Our extensive cDNA and ORF collections are available as individual clones or in various library formats, tailored to suit diverse research needs.

 
  • Mammalian Gene Collection (MGC)

Recognized as the gold standard for full-length cDNA sequences, the Mammalian Gene Collection offers fully sequenced cDNA clones for human, mouse, rat, and bovine genes.

  • CCSB-Broad Lentiviral Expression Library

This library provides human ORFs in lentiviral expression vectors equipped with a C-terminal V5 tag, ideal for protein expression and interaction studies.

  • ORFeome Collaboration

The ORFeome Collaboration offers sequence-verified human ORFs subcloned into Gateway® recombinational entry vectors, allowing seamless integration into various experimental systems.

  • CCSB hORFeome Libraries

Version 8.1: The latest release of the CCSB Human ORFeome library, developed by the Dana-Farber Cancer Institute, offering unparalleled coverage and precision.

Non-Mammalian cDNA & ORFs

Expand your research capabilities with our range of cDNA and ORF clones for non-mammalian species, including:

  • Yeast
  • E. coli
  • C. elegans
  • D. rerio (Zebrafish)
  • Xenopus (Frog)

We are in the process of updating our website to include a complete list of available species and clones. In the meantime, feel free to contact us directly with your specific requests or inquiries.


Please don’t hesitate to Send Us A Message. We’ll get back to you with a tailored quote as soon as possible.

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