Search your gene of interest to find cDNA, ORF, and Lentiviral ORF Clones
SkyElite™ cDNA and ORF Clones
Full-length, sequence guaranteed clones for gene overexpression
SkyElite™ cDNA and ORF clones offers a dependable way to accelerate your research and improve experimental success. Our clones are derived from the Mammalian Gene Collection (MGC) from the NIH, with a proven sequence accuracy of less than 1 error in 50,000 base pairs. Each clone is guaranteed to match the published sequence. There is a small possibility of mistaken identification, incorrect DNA sequence, or incorrect annotation. Researchers are advised to perform end-sequencing of their purchased MGC clone and BLAST the results against the published GenBank sequence. This can also be accomplished through Skyang’s sequence verification service. If there is any problem with the clone, we will replace with an alternate clone when available or provide a refund.
For added confidence, we offer an optional end-sequencing verification service to confirm clone identity before shipping, ensuring accurate and risk-free results.
Deliverable: Glycerol stock in 2ml tube
Timeline: Shipped within 48 hours for standard glycerol stock. 1 week for the sequence verification option.
SkyElite cDNA and ORF clones can be conveniently ordered through Skyang gene search tool. Choose “pick and ship” for quick delivery or choose sequence verification for the clone to be end-sequenced verified prior to shipment. Sequence information will be included with the C of A.
Human cDNA Clones
Includes the open reading frame and untranslated regions (UTR)
- Retain native regulatory elements, allowing the study of genes in their natural regulatory context.
- Ideal for applications where preserving endogenous gene regulation is critical, such as transcriptional control studies.
Human ORF Clones
Excludes 5′ and 3′ untranslated regions (UTRs) Gateway© adapted vector
- Provides a streamlined approach for applications requiring efficient protein production.
- Direct and simplified route to protein expression
Human LentiORF Clones
Lentiviral ORF expression vectors equipped with a V5 tag in the pLX304 vector
- Efficient and stable gene delivery to a wide range of cell types, including both dividing and non-dividing cells
- Enables creation of stably expressing cell lines for gene overexpression and functional studies
SkyElite cDNA and ORF Libraries
High quality, fully sequenced genome-scale cDNA and ORF collections
Skyang Bio’s (Formerly Transomic Technologies) SkyElite™ cDNA and ORF arrayed libraries are genome-scale collections developed using the most rigorous sequencing process ensuring the highest quality and confidence. However, you should expect to see some minor rates of error. This is inherent with any large-scale resource created by multiple labs using multiple strategies and pipelines for cloning, arraying, sequencing, and analyses. It is always best to practice clonal isolation and sequence verification for each clone prior to use. Due to the nature of arrayed format, even when best practices are followed, there is the possibility of mixed populations in wells.
Exceptional academic pricing available
SkyElite Human and Mouse cDNA Whole Genome Libraries
The SkyElite cDNA Library is a comprehensive, genome-scale collection representing genes from human and mouse. The aim is to provide researchers with sequence-validated, full-length, protein-coding (FL-CDS) cDNA clones to aid in gene function studies and other biomedical research. The human cDNA library represents 15,928 genes with 26,806 clones. The mouse cDNA library represents 15,836 genes with 23,838 clones. Sub libraries containing only the pCMV-SPORT6 expression-ready clones are also conveniently available.
Human cDNA Library – Cat# SECH3500 – Logistics: Delivered as Glycerol Stock in 100 (384-well plate format) and 295 (96-well plate format)
Mouse cDNA Library – Cat# SECM3500 – Logistics: Delivered as Glycerol Stock in 297 (96-well plate format)
SkyElite Human ORFeome Collaboration Library
The SkyElite human ORF library includes full-length, annotated and sequence verified ORFs and have been transferred into Gateway™ Entry vectors as a ready-to-use resource for recombinant protein expression. The human ORF library represents 9804 genes with 16,581 clones.
Cat# SEOH3507 – Logistics: Delivered as Glycerol Stock in 196 (96-well plate format)
SkyElite Human ORFeome Collection v9
The SkyElite v9 ORF human ORF library has been verified by next generation sequencing and is provided in a Gateway™ adapted entry vector for fast and convenient transfer to any compatible expression vector. The human v9 ORF library represents 11,016 genes with 12,071 clones.
Cat# SEOH3506 – Logistics: Delivered as Glycerol Stock in 132 (96-well plate format)
SkyElite Human Lentiviral ORF Expression Library
The SkyElite human Lentiviral ORF library provides the most fully sequenced and annotated ORF collection available. The lentiviral ORF expression vector was created to enable expression of a protein of interest with a V5 fusion tag for western blot detection, purification, co-immunoprecipitation, protein localization and FACS analysis.
Cat# SELOH7500 – Logistics: Delivered as Glycerol Stock in 169 (96-well plate format)
Timeline: 4-6 weeks upon receipt of order
Non-Mammalian cDNA and ORF Clones
Expand your research capabilities with our range of cDNA and ORF clones for non-mammalian species, including:
- Yeast
- E. coli
- C. elegans
- D. rerio (Zebrafish)
- Xenopus (Frog)
Contact Us for more information about non-mammalian clones
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