NGS and Deconvolution
ServiceFrom pooled libraries to clear research results
Skyang Bio can help analyze results from your CRISPR or shRNA pooled screening experiments. Send us cell pellets from your screen and we will extract genomic DNA, prepare the samples for Next-Generation Sequencing (NGS), perform NGS on the appropriate NGS platform, and align the gRNA/shRNA sequence reads against the NGS reads from the screening pool. You can then determine which gRNAs/shRNAs in your screening pool were enriched or depleted.
- Skyang offers the highest quality CRISPR/shRNA screening libraries for your experiment
- Designed to support discovery, functional genomics, and screening studies
- High-accuracy sequencing with flexible read lengths and depths
- Flexible library preparation for CRISPR and RNAi pooled libraries
- Perform your screens on your cell lines
- Send frozen cell pellets to Skyang
- We will extract gDNA and prepare the samples for NGS
- Skyang will run the samples on the appropriate NGS platform
Deliverable:
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- Data comparing the gRNA/shRNA NGS counts of the screening pool compared to the gRNA/shRNA counts from the screened samples
Timeline: 1 week per sample
Fill out our quick request form and
our team will prepare a detailed quote for you
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