Skyang Bio Service: POOL AND ARRAYED LIBRARY
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Pooled CRISPR screens are only as effective as the quality of the libraries used. At Skyang Bio, we specialize in creating custom CRISPR libraries that are tailored to your specific research needs. Whether you’re looking to perform CRISPR knockout (CRISPRko), activation (CRISPRa), or interference (CRISPRi) screens, we offer cutting-edge solutions that support your experimental goals with precision and efficiency.
Our expertly designed CRISPR pooled and arrayed libraries ensure reproducibility and high screening quality for a wide range of applications.
Ready to take your research to the next level? Contact Skyang Bio today to discuss how we can help you achieve your project’s goals with our custom CRISPR technologies.
Highlights
Customer Screening pools
Customers can use any of our CRISPR or shRNA lentiviral vectors for creating custom screening pools.
- Client identifies genes of interest to be targeted (i.e. druggable genes) and desired vector
- Skyang uses its gRNA algorithm or shRNA algorithm to design targeting gRNAs or shRNA
- Skyang synthesizes gRNAs or shRNAs and clones into vector backbone
- The screening pool is qualified by Next-Gen Sequencing to ensure equal representation of every construct.
- Skyang packages the screening pool into viral particles and performs functional titer analysis.
Customer Screening Pools QC
Skyang Bio offers a Certificate of Analysis for all screening pools, showing distribution of gRNAs/shRNAs as determined by Nex–Gen Sequencing. 
Lentiviral pools will be evaluated by functional viral titer*.
*Skyang Bio performs a functional titer on all lentiviral particles delivered. Functional titer analysis (opposed to physical titer analysis) is a more accurate measurement for calculating MOIs that are used in screening experiments.
Customer Arrayed Library
Streamlined work-flows allow Skyang to be one of the few vendors that offers customized arrayed CRISPR and shRNA libraries in 96-well format