cDNA
MGC premier cDNA collections offer a convenient solution that decreases the time required to start an experiment and increases the chances of success. Creating cDNA clones from biological material requires time, labor and money. While cDNA clone production is a well-established process it requires three major steps: synthesis, cloning and validation. Rather than spending time on cloning, simply search for your gene of interest using search tool.
cDNA clones:Full length, sequence guaranteed cDNA clones
Product Highlights:
- cDNA clones include open reading frame and untranslated regions (UTR)
- Human, mouse, rat, bovine, Xenopus and zebrafish genomes represented
- Available as individual clones, gene family and pathway libraries, whole genome libraries and custom rearrays
- Multiple expression-ready cloning vectors available to create your expression-ready cDNA
- Custom cloning and lentiviral packaging services available
Based on the Mammalian Gene Collection (MGC) developed by the National Institutes of Health (NIH) with rigorous sequence analysis resulting in less than 1 error in 50,000 bp (MGC Project Team 2009). All MGC premier cDNA clones are backed by a 100% guarantee to be an exact match to the published sequence.
Clone isolation and end-sequencing option
Want to confirm the identity of your cDNA before shipping? We can streak isolate a colony and end sequence it before delivery. Select the end-sequenced format when choosing your cDNA from search tool. This risk free option ensures you start with a verified clonal culture.
cDNA Libraries
MGC Premier cDNA Libraries
MGC premier cDNA Libraries are genome-scale collections for human and mouse species. These collection are derived from the MGC collection developed by the National Institutes of Health (NIH) using the most rigorous sequencing process ensuring the highest sequence quality and confidence.
MGC Premier cDNA gene family and pathway libraries
MGC premier cDNA gene families and pathways are arrayed in to focus sets making screening possible for large and small laboratories. The libraries are arrayed from the MGC premier cDNA collection cloned into the pCMV-SPORT6 vector.
The gene lists were created using resources from the National Institute of Health’s Cancer Genome Anatomy Project (CGAP) and Gene Ontology (GO). Annotation is based on electronic and manual literature-based curation as well as sequence analysis. (See Annotation Standard Operating Procedures from the Gene Ontology Consortium for more information.)
**Gene Family and Pathway libraries are limited by the availability of MGC clones in pCMV-SPORT6.
Collections are shipped in 96 well plate format on dry ice. Individual clones and collections should be stored at –80°C immediately on receipt.