SkyEDIT™ CRISPR gRNA
Target Gene SetsOptimized gRNA designs, versatile vectors and flexible formats for efficient gene editing
SkyEDIT CRISPR-Cas9 gRNA Target gene sets are available in a range of vectors including CRISPRko (knockout) single gRNA or gRNA+Cas9, CRISPRa (activation), and CRISPRi (interference). The sgRNA vectors are designed to select for high gene-editing efficiency using direct indicators of CRISPR-Cas9 expression from fluorescent reporters or selection markers. The Croatan algorithm was used to design all gRNAs or we can use your specific gRNA designs upon request.
- CRoatan algorithm gRNA designs result in superior knockout efficiency
- sgRNA lentiviral vectors include versatile promoter, selection marker and fluorescent reporter options
- All clones are sequence-verified
Deliverable:
- 3 unique gRNA constructs targeting your gene of interest
- Negative control
- Glycerol stock, Lentiviral Particles. or Plasmid DNA
Timeline: 2-3 weeks for glycerol stock. Additional 2 weeks for lentiviral particles
Price: Starting at $775 for glycerol stock
Request a quote for CRISPR gRNA target gene set
targeting your gene of interest
Available Vectors
Single gRNA Vector Options
All-in-one gRNA + Cas9 Vector Options
CRISPRa gRNA Vector Options
CRISPRi gRNA Vector Options
CRISPR Dual gRNA Vector Options
CRISPR ccdB Cloning Vector
The ccdB cassette is inserted between the U6 promoter and the gRNA, which is removed upon insertion of the target gRNA. Because the ccdB vector cannot survive in standard competent cells used for sgRNA cloning, this design significantly minimizes background contamination and improves the efficiency of obtaining correct gRNA clones.
This CRISPR ccdB cloning vector is ideal for researchers who need reliable, high-throughput-compatible CRISPR cloning to support both targeted experiments and large-scale genetic studies.
Gene knockout/knock-in studies, pathway analysis, and functional validation
Interrogation of gene function in cellular processes and phenotypic assays
Targeted gene perturbation studies and CRISPR-based screens
Identification and validation of oncogenes, tumor suppressors, and drug targets
Gene editing in immune cells to study signaling, activation, and immune responses
Studying gene regulation during differentiation
Genome engineering in pluripotent or differentiated stem cell models
SkyEDIT™ Dual gRNA Whole Genome Arrayed Library
Dual gRNA lentiviral vector developed using the CRoatan gRNA design algorithm for superior knockout efficiency
Skyang Bio’s SkyEDIT™ dual gRNA CRISPR arrayed library was developed in collaboration with Cold Spring Harbor Laboratory. The dual gRNA design increases the likelihood of CRISPR Cas9 cuts and frameshifts for efficient gene knockout. Each lentiviral construct expresses 2 gRNAs targeting the same gene, where all gRNAs have been designed using the CRoatan algorithm (read publication). Each dual gRNA construct contains a unique barcode, a puromycin selection marker and a GFP fluorescent reporter
- Over 18,000 human genes targeted
- Each human gene is targeted by 3-5 unique dual gRNA constructs
- Every dual gRNA construct has been sequence-verified
- Over 1,000 negative controls are contained in the arrayed library
Deliverable:
- 670 96-well glycerol stock plates with barcoded labels
- Datafile for 96-well plate clone mapping
- Pooled format available as lentiviral particles or plasmid DNA
Timeline: 4-6 weeks
Library comes in lentiviral vector that contains the SFFV promoter with a puromycin selection marker and a GFP fluorescent reporter
Request a quote for CRISPR Dual gRNA
Whole Genome Arrayed Library
Need a custom screening library cloned into one of our available vectors or a vector you provide to us? We offer custom pooled and arrayed libraries to suit your specific need. Visit Custom Pooled and Arrayed Screening Libraries or Contact us at products@skyangbio.com for more information
Documents
Related Products
Custom CRISPR gRNA pooled and arrayed screening libraries – We can build custom CRISPR pooled libraries for your screening projects and genome editing experiments. Stringent quality control is maintained throughout our process. All pools go through a Next-Gen sequencing QC to confirm all constructs in the screening pool are represented in an equal manner. The CRoatan algorithm is used to design gRNAs. Other gRNA design algorithms available upon request.
Cloning Vectors – Our cloning vectors provide robust modular flexibility, allowing the incorporation of various molecular tags and antibiotic-resistant genes.
Custom Vector Construction – Skyang will engineer your next custom vector. Choose from our highly customizable inventory of lentiviral and retroviral cloning vectors. If you need a vector that we currently do not offer, then we can generate that vector for you.
Lentiviral Packaging Service – Skyang lentiviral packaging workflow produces high-quality lentiviral particles. All lentiviral particles are functionally titered, which is the gold standard for quality control. This facilitates the most precise calculation of MOI for optimized transduction.
Custom NGS and Deconvolution Service – Skyang can help analyze results from your CRISPR pooled screening experiments.