Dual gRNA lentiviral vector developed using the CRoatan gRNA design algorithm for superior knockout efficiency

SkyangBio’s dual gRNA CRISPR library was developed in collaboration with Cold Spring Harbor Laboratory. The library targets over 18,000 human genes and is the only dual gRNA arrayed library in the industry. The CRISPR library is conveniently delivered as glycerol stock clones in 96-well plate format.

Features

• Over 18,000 human genes targeted
• Each human gene is targeted by 3-5 unique dual gRNA constructs
• Every dual gRNA construct has been 100% sequence-verified
• Over 1,000 negative controls are contained in the arrayed library
• Each dual gRNA construct contains a unique barcode, a puromycin selection marker and a GFP fluorescent reporter

Advantages

• The Croatan algorithm was used to design all gRNAs. Read the publication in Molecular Cell
• The dual gRNA design increases the likelihood of CRISPR Cas9 cuts and frameshifts for efficient gene knockout
• Lentiviral vector packages efficiently for high-titer viral particles
• All CRISPR library constructs are 100% sequence-confirmed
• Glycerol stock 96-well plates are easily maintained and offer convenient work-flow

Deliverable

• 670 96-well glycerol stock plates with barcoded labels
• 100% sequence-confirmed dual gRNA constructs
• Datafile for 96-well plate clone mapping