shERWOOD shRNA design + Optimized shRNA processing = Superior Knockdown

SkyangBio’s shERWOOD-UltramiR™ shRNA libraries are next-generation, vector-based RNAi triggers designed using the proprietary shERWOOD algorithm that was developed and validated in Dr. Gregory Hannon’s laboratory at Cold Spring Harbor Laboratory (Knott et al 2014). The library uses an alternate microRNA scaffold called “UltramiR“. The UltramiR scaffold has been optimized for increased shRNA processing and potency based on the key determinants for primary microRNA processing (Auyeung et al 2013).

The shERWOOD algorithm is based on the functional testing of over 250,000 shRNA sequences using a high-throughput sensor assay and uses key sequence characteristics for predicting shRNA potency to select the rare shRNA designs that are potent at single copy representation in the genome.

Features

• Over 18,000 mouse genes targeted
• Each human gene is targeted by an average of 5 shRNA constructs
• The shRNAs are cloned into a retroviral vector that contains a Neomycin selection marker and a ZsGreen fluorescent reporter

Advantages

• The proprietary shERWOOD algorithm was used to design all shRNA. Read the publication in Molecular Cell
• The UltramiR microRNA scaffold increases shRNA processing and potency
• All mouse shRNA library constructs are 100% sequence-confirmed
• Glycerol stock 96-well plates are easily maintained and offer a convenient workflow

Deliverables

• Human shRNA library: 898 96-well glycerol stock plates with barcoded labels
• Data file for 96-well plate clone mapping